Microcomputed Tomography and Microfinite Element Modeling for Evaluating Polymer Scaffolds Architecture and Their Mechanical Properties

被引:29
作者
Alberich-Bayarri, Angel [2 ]
Moratal, David [3 ]
Escobar Ivirico, Jorge L. [3 ,5 ]
Rodriguez Hernandez, Jose C. [3 ,5 ]
Valles-Lluch, Ana [3 ]
Marti-Bonmati, Luis [2 ]
Mas Estelles, Jorge [3 ]
Mano, Joao F. [1 ]
Monleon Pradas, Manuel [3 ,4 ,5 ]
Gomez Ribelles, Jose L. [3 ,4 ,5 ]
Salmeron-Sanchez, Manuel [3 ,4 ,5 ]
机构
[1] Univ Minho, Dept Polymer Engn, Res Grp Biomat Biodegradables & Biomimet 3Bs, P-4710057 Braga, Portugal
[2] Hosp Quiron, Dept Radiol, Valencia 46010, Spain
[3] Univ Politecn Valencia, Ctr Biomat & Tissue Engn, Valencia 46022, Spain
[4] Ctr Invest Principe Felipe, Valencia 46013, Spain
[5] Networking Res Ctr Bioengn Biomat & Nanomed CIBER, Valencia, Spain
关键词
scaffolds; mechanical properties; finite element analysis; tissue engineering; COMPUTED-TOMOGRAPHY; MICRO-CT; BONE ARCHITECTURE; CELLULAR SOLIDS; TISSUE; FABRICATION; PORES; MICROSTRUCTURE; INGROWTH; DESIGN;
D O I
10.1002/jbm.b.31389
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Detailed knowledge of the porous architecture of synthetic scaffolds for tissue engineering, their mechanical properties, and their interrelationship was obtained in a nondestructive manner. Image analysis of microcomputed tomography (mu CT) sections of different scaffolds was done. The three-dimensional (3D) reconstruction of the scaffold allows one to quantify scaffold porosity, including pore size, pore distribution, and struts' thickness. The porous morphology and porosity as calculated from mu CT by image analysis agrees with that obtained experimentally by scanning electron microscopy and physically measured porosity, respectively. Furthermore, the mechanical properties of the scaffold were evaluated by making use of finite element modeling (FEM) in which the compression stress-strain test is simulated on the 3D structure reconstructed from the mu CT sections. Elastic modulus as calculated from FEM is in agreement with those obtained from the stress-strain experimental test. The method was applied on qualitatively different porous structures (interconnected channels and spheres) with different chemical compositions (that lead to different elastic modulus of the base material) suitable for tissue regeneration. The elastic properties of the constructs are explained on the basis of the FEM model that supports the main mechanical conclusion of the experimental results: the elastic modulus does not depend on the geometric characteristics of the pore (pore size, interconnection throat size) but only on the total porosity of the scaffold. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 91B: 191-202, 2009
引用
收藏
页码:191 / 202
页数:12
相关论文
共 49 条
[1]   Trabecular surface remodeling simulation for cancellous bone using microstructural voxel finite element models [J].
Adachi, T ;
Tsubota, K ;
Tomita, Y ;
Hollister, SJ .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 2001, 123 (05) :403-409
[2]  
[Anonymous], ADV POLYM SCI
[3]   Bulk and interface investigations of scaffolds and tissue-engineered bones by X-ray microtomography and X-ray microdiffraction [J].
Cancedda, R. ;
Cedola, A. ;
Giuliani, A. ;
Komlev, V. ;
Lagomarsino, S. ;
Mastrogiacomo, M. ;
Peyrin, F. ;
Rustichelli, F. .
BIOMATERIALS, 2007, 28 (15) :2505-2524
[4]   3D microtomographic characterization of precision extruded poly-ε-caprolactone scaffolds [J].
Darling, AL ;
Sun, W .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2004, 70B (02) :311-317
[5]   Polymer scaffolds with interconnected spherical pores and controlled architecture for tissue engineering:: Fabrication, mechanical properties, and finite element modeling [J].
Diego, Raul Brigido ;
Estelles, Jorge Mas ;
Sanz, Jose Antonio ;
Garcia-Aznar, Jose Manuel ;
Sanchez, Manuel Salmeron .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2007, 81B (02) :448-455
[6]   Pore collapse during the fabrication process of rubber-like polymer scaffolds [J].
Diego, Raul Brigido ;
Gomez Ribelles, Jose Luis ;
Sanchez, Manuel Salmeron .
JOURNAL OF APPLIED POLYMER SCIENCE, 2007, 104 (03) :1475-1481
[7]   Guidance of engineered tissue collagen orientation by large-scale scaffold microstructures [J].
Engelmayr, George C., Jr. ;
Papworth, Glenn D. ;
Watkins, Simon C. ;
Mayer, John E., Jr. ;
Sacks, Michael S. .
JOURNAL OF BIOMECHANICS, 2006, 39 (10) :1819-1831
[8]   Structure and properties of methacrylate-endcapped caprolactone networks with modulated water uptake for biomedical applications [J].
Escobar Ivirico, J. L. ;
Costa Martinez, E. ;
Salmeron Sanchez, M. ;
Munoz Criado, I. ;
Gomez Ribelles, J. L. ;
Monleon Pradas, M. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2007, 83B (01) :266-275
[9]   THE DIRECT EXAMINATION OF 3-DIMENSIONAL BONE ARCHITECTURE INVITRO BY COMPUTED-TOMOGRAPHY [J].
FELDKAMP, LA ;
GOLDSTEIN, SA ;
PARFITT, AM ;
JESION, G ;
KLEEREKOPER, M .
JOURNAL OF BONE AND MINERAL RESEARCH, 1989, 4 (01) :3-11
[10]   Chondrogenesis in a cell-polymer-bioreactor system [J].
Freed, LE ;
Hollander, AP ;
Martin, I ;
Barry, JR ;
Langer, R ;
Vunjak-Novakovic, G .
EXPERIMENTAL CELL RESEARCH, 1998, 240 (01) :58-65