Assessment of Capsaicinoid and Capsinoid Accumulation Patterns during Fruit Development in Three Chili Pepper Genotypes (Capsicum spp.) Carrying Pun1 and pAMT Alleles Related to Pungency

被引：30
作者：
Fayos, Oreto [1 ]
Ochoa-Alejo, Neftali [2 ]
Martinez de la Vega, Octavio [3 ]
Saviron, Maria [4 ]
Orduna, Jesus [4 ]
Mallor, Cristina [1 ]
Barbero, Gerardo F. [5 ]
Garces-Claver, Ana [1 ]
机构：
[1] Univ Zaragoza, Unidad Hortofruticultura, Ctr Invest & Tecnol Agroalimentaria Aragon, CITA,Inst Agroalimentario Aragon IA2, Avda Montanana 930, E-50009 Zaragoza 50059, Spain
[2] Inst Politecn Nacl, Unidad Irapuato, Ctr Invest & Estudios Avanzados, Dept Ingn Genet, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Irapuato, Gto, Mexico
[3] Inst Politecn Nacl, Unidad Genom Avanzada UGA LANGEBIO, Ctr Invest & Estudios Avanzados, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Irapuato, Gto, Mexico
[4] Univ Zaragoza, CSIC, Fac Ciencias, Inst Ciencia Mat Aragon ICMA CEQMA, C Pedro Cerbuna 12, E-50009 Zaragoza, Spain
[5] Univ Cadiz, Dept Analyt Chem, Fac Sci, IVAGRO,Agrifood Campus Int Excellence CeiA3, POB 40, Cadiz 11510, Spain
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 44期
关键词：
Capsicum spp; fruit development; accumulation pattern; pungency genes; HPLC-ESI-MS(QTOF); PUTATIVE-AMINOTRANSFERASE; CH-19; SWEET; ANNUUM; BIOSYNTHESIS; ANALOGS; DIHYDROCAPSIATE; IDENTIFICATION; SUBSTANCES; EVOLUTION; CAPSIATE;
D O I：
10.1021/acs.jafc.9b05332
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Quantification, using an accurate analytical approach, of capsinoids and capsaicinoids was performed on three chili pepper (Capsicum spp.) genotypes: "Chiltepin", "Tampiquerio 74", and "Bhut Jolokia" at various stages of fruit development. The accumulation of capsinoids, in all these peppers started between 10 to 20 days post-anthesis (dpa), increased and reached the highest capsinoid amount at 40 dpa, and then decreased until 60 dpa. Conversely, capsaicinoids could already be determined at 10 dpa in "Bhut Jolokia" and their accumulation pattern was different from that of the capsinoids in this genotype. The capsiate/dihydrocapsiate ratio presented a higher variation between genotypes and developmental stages than the capsaicin/dihydrocapsaicin ratio. Capsinoid ratios (4-24%) and Pun1/pAMT genotyping were determined. These results provide information on the progress of the accumulation of capsinoids in the aforementioned pungent and superhot cultivars and could support future breeding studies toward the understanding of the factors affecting their accumulation.
引用
收藏
页码：12219 / 12227
页数：9
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