Differential microRNA expression profiling of mesothelioma and expression analysis of miR-1 and miR-214 in mesothelioma

被引:31
作者
Amatya, Vishwa Jeet [1 ]
Mawas, Amany Sayed [1 ,2 ]
Kushitani, Kei [1 ]
El-Din, Mouchira M. Mohi [2 ]
Takeshima, Yukio [1 ]
机构
[1] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Pathol, Hiroshima 7348551, Japan
[2] South Valley Univ, Fac Vet Med, Dept Pathol & Clin Pathol, Qena, Egypt
基金
日本学术振兴会;
关键词
mesothelioma; microRNA; miR-1; miR-214; PIM1; functional assay; DOWN-REGULATION; PROTOONCOGENE PIM-1; PROGNOSTIC MARKER; LUNG-CANCER; OVEREXPRESSION; METASTASIS; APOPTOSIS; SOFTWARE; SURVIVAL;
D O I
10.3892/ijo.2016.3358
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Malignant mesothelioma is a highly aggressive cancer with poor prognosis and refractory to currently available therapies. Most of the patients with advanced invasive nature are not amenable to surgical resection and/or available anticancer therapy, thus development of novel effective therapeutic regimes is needed. Aberrant expression of microRNAs (miRNAs) has been proposed to contribute to carcinogenesis and aggressiveness of mesothelioma. We analyzed miRNA expression in mesothelioma cell lines using TaqMan miRNA expression array and found significant number of miRNAs, which showed increased or lost expression. We validated the increased expression of miR-182, and miR-183 in mesothelioma cell lines by individual miRNA assays and SmartFlare miRNA probes. We further investigated the miR-1, and miR-214, which were not expressed in mesothelioma cells by real-time RT-PCR. Transfection of mesothelioma cells, ACC-Meso-1 and CRL5915, with miRNA mimic (hsa-miR-1 mimic and hsa-miR-214 mimic) led to inhibition of cell growth, invasion and migration. We paid attention to PIM1, the target gene of both miR-1 and miR-214 miRNAs and which was found overexpressed in mesothelioma cells, and miR-1 and miR-214 mimic transfection of mesothelioma cell lines showed downregulation of PIM1 by western blot analysis. The miRNAs, miR-1 and miR-214, may play a role in carcinogenesis of mesothelioma thus might be considered as candidate therapeutic targets in mesothelioma.
引用
收藏
页码:1599 / 1607
页数:9
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