Development and Validation of a HPLC Method for the Estimation of Omeprazole in Human Plasma

被引:0
作者
Lou, Jie [1 ]
Yu, Linzhi [2 ]
Li, Jianyang [1 ]
Liu, Jianping [1 ]
Ding, Quanhua [1 ]
Chen, Wujie [1 ]
Sun, Lingjia [1 ]
Zhang, Youping [1 ]
机构
[1] Ningbo 2 Hosp, Dept Gastroenterol, Ningbo 315000, Zhejiang, Peoples R China
[2] Ningbo 2 Hosp, Endoscopy Ctr, Ningbo 315000, Zhejiang, Peoples R China
来源
LATIN AMERICAN JOURNAL OF PHARMACY | 2016年 / 35卷 / 02期
关键词
HPLC; omeprazole; pharmacokinetic study; plasma; PROTON PUMP INHIBITORS; CAPILLARY-ELECTROPHORESIS; PHARMACOKINETICS; LANSOPRAZOLE; PANTOPRAZOLE; METABOLITES; RABEPRAZOLE; CYP2C19; ASSAY; LIQUID;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
In this study, a simple, rapid and sensitive high performance liquid chromatography (HPLC) method is developed for the determination of omeprazole in human plasma samples using ketoconazole as the internal standard (IS). Sample preparation was accomplished through one-step liquid-liquid extraction with ethyl acetate, and chromatographic separation was carried out on an Agilent ZORBAX Eclipse XDB-C18 (4.6 x 150 mm, 5 mu m) at 30 degrees C. Mobile phase composed of a mixture of acetonitrile-0.1% trifluoroacetic acid-water (20: 10: 70) at flow rate of 1.0 mL/min. Wavelength was set at 302 nm. The chromatographic retention times of omeprazole and IS were 5.61 and 4.17 min, respectively. The lower limit of quantitation (LLOQ) was 25 ng/mL, and no interferences were detected in the chromatograms. The devised HPLC method was validated by evaluating its intra-and inter-day precisions and accuracies in a linear concentration range between 25 and 1500 ng/mL. The method was successfully applied to a pharmacokinetic study of oral omeprazole enteric coated tablets in Chinese healthy volunteers.
引用
收藏
页码:239 / 243
页数:5
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