High-Performance Liquid Chromatography Using UV Detection for the Simultaneous Quantification of Ropivacaine and Bupivacaine in Human Plasma

被引：13

作者：

Gaudreault, Francois ^{[1
]}

Drolet, Pierre ^{[2
]}

Varin, France ^{[1
]}

机构：

[1] Univ Montreal, Fac Pharm, Quebec City, PQ H3T 1J4, Canada

[2] Hop Maison Neuve Rosemont, Dept Anesthesie Reanimat, Montreal, PQ H1T 2M4, Canada

来源：

THERAPEUTIC DRUG MONITORING | 2009年 / 31卷 / 06期

关键词：

ropivacaine; bupivacaine; HPLC; perioperative context; MASS-SPECTROMETRY; LOCAL-ANESTHETICS; POSTOPERATIVE ANALGESIA; NERVE BLOCKADE; KNEE SURGERY; HUMAN SERUM; PHARMACOKINETICS;

D O I：

10.1097/FTD.0b013e3181bc8014

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A specific high-performance liquid chromatography assay coupled with UV detection has been developed and validated for the simultaneous determination of ropivacaine and bupivacaine in human plasma. A liquid-liquid back extraction procedure was used to increase specificity, and very good and consistent recoveries were obtained: 93%-95% for ropivacaine and 90%-96% for bupivacaine. The lowest limit of quantification was 4 and 8 ng/mL for ropivacaine and bupivacaine, respectively The method was sensitive, reproducible (coefficient of variation <= 6.9%), and accurate (96%-102%) and was applied successfully to study the pharmacokinetics of ropivacaine and bupivacaine in a perioperative context.

引用

页码：753 / 757

页数：5

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