Effects of Piper sarmentosum Water Extract on 11-β Hydroxysteroid Dehydrogenase Type 1 Bioactivity in Ovariectomy-Induced Obese Rats

被引:0
作者
Azlina, A. Aida [1 ]
Farihah, H. S. [2 ]
Qodriyah, H. M. S. [1 ]
Azlina, M. F. Nur [1 ]
机构
[1] Univ Kebangsaan Malaysia, Dept Pharmacol, Fac Med, Kuala Lumpur 50300, Malaysia
[2] Univ Kebangsaan Malaysia, Dept Anat, Fac Med, Kuala Lumpur 50300, Malaysia
关键词
Piper sarmentosum; obese; 11; beta-HSD1; ovariectomy; body weight; HEPATIC INSULIN SENSITIVITY; 11-BETA-HYDROXYSTEROID DEHYDROGENASE; BODY-COMPOSITION; ADIPOSE-TISSUE; IN-VIVO; SELECTIVE-INHIBITION; CORTISOL METABOLISM; MESSENGER-RNA; EXPRESSION; LOCALIZATION;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) convert inactive circulating 11-keto steroids into active glucocorticoids, amplifying local glucocorticoid action. It is elevated in adipose tissue in obese humans and rodents, suggesting that adipose tissue glucocorticoid excess may be the causative factor for obesity. This study was conducted to evaluate the effects of Piper sarmentosum (PS) water extract and glycyrrhizic acid (GCA) on 11 beta-HSD1 bioactivity in ovariectomized induced obese rats. Forty-two female Sprague-Dawley rats were randomly divided into six groups; four treatments (PS, GCA, CTRL and SHM) and two basal (B-CTRL and B-SHM). All groups underwent ovariectomy excluding SHM and B-SHM which underwent sham operation. Basal groups were sacrificed on the first day of treatment, while ovariectomized groups were given PS extract (0.125 g kg(-1)), GCA (0.120 g kg(-1)) and water (CTRL), respectively, while SHM received only water. Blood pressure was measured monthly while body weight weekly. After five months, rats were sacrificed and liver, heart and visceral adipose tissues were taken for analysis. Piper sarmentosum (PS) and GCA group showed a significant reduction in enzyme activity but no difference in body weight compared to CTRL group. Meanwhile only the blood pressure in GCA group was significantly higher after three months of treatment as compared to CTRL group but no difference after five months. In conclusion, both PS water extract and GCA have the ability to reduce 11 beta-HSD1 enzyme activity but only GCA cause an increased in blood pressure.
引用
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页码:362 / 369
页数:8
相关论文
共 41 条
[1]  
AGARWAL AK, 1989, J BIOL CHEM, V264, P18939
[2]   Selective inhibition of 11β-hydroxysteroid dehydrogenase type 1 improves hepatic insulin sensitivity in hyperglycemic mice strains [J].
Alberts, P ;
Nilsson, C ;
Selén, G ;
Engblom, LOM ;
Edling, NHM ;
Norling, S ;
Klingström, G ;
Larsson, C ;
Forsgren, M ;
Ashkzari, M ;
Nilsson, CE ;
Fiedler, M ;
Bergqvist, E ;
Öhman, B ;
Björkstrand, E ;
Abrahmsén, LB .
ENDOCRINOLOGY, 2003, 144 (11) :4755-4762
[3]   Selective inhibition of 11β-hydroxysteroid dehydrogenase type 1 decreases blood glucose concentrations in hyperglycaemic mice [J].
Alberts, P ;
Engblom, L ;
Edling, N ;
Forsgren, M ;
Klingström, G ;
Larsson, C ;
Rönquist-Nii, Y ;
Öhman, B ;
Abrahmsén, L .
DIABETOLOGIA, 2002, 45 (11) :1528-1532
[4]  
ALWAHAIBI A, 2007, TROP MED PLANTS, V8, P21
[5]   Glycyrrhetinic acid, the active principle of licorice, can reduce the thickness of subcutaneous thigh fat through topical application [J].
Armanini, D ;
Nacamulli, D ;
Francini-Pesenti, F ;
Battagin, G ;
Ragazzi, E ;
Fiore, C .
STEROIDS, 2005, 70 (08) :538-542
[6]   Role of glucocorticoids in the physiopathology of excessive fat deposition and insulin resistance [J].
Asensio, C ;
Muzzin, P ;
Rohner-Jeanrenaud, F .
INTERNATIONAL JOURNAL OF OBESITY, 2004, 28 (Suppl 4) :S45-S52
[7]  
AZLINA AA, 2009, RES J MED P IN PRESS
[8]   Peroxisome proliferator-activated receptor-γ ligands inhibit adipocyte 11β-hydroxysteroid dehydrogenase type 1 expression and activity [J].
Berger, J ;
Tanen, M ;
Elbrecht, A ;
Hermanowski-Vosatka, A ;
Moller, DE ;
Wright, SD ;
Thieringer, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (16) :12629-12635
[9]  
BJORNTORP P, 1991, INT J OBESITY, V15, P67
[10]   Light and electron microscopy localization of the 11ß-hydroxysteroid dehydrogenase type I enzyme in the rat [J].
Brereton, PS ;
van Driel, RR ;
Suhaimi, FBH ;
Koyama, K ;
Dilley, R ;
Krozowski, Z .
ENDOCRINOLOGY, 2001, 142 (04) :1644-1651