Kinetics and morphology analysis for fabrication a mimetic bio-membrane on Brij-76 derived surface

We investigated the effects of the phosphoglycerides (L-alpha-dipalmitoyl-phosphatidylcholine (DPPC) and lecithin (egg PC)) and cholesterol contents of liposome on the topographies and the kinetics deposition mechanisms on the polyethylene glycol ocatadecyl ether (Brij-76) derived solid surface by (1) the morphology analysis of atomic force microscopy and (2) the real-time monitoring of quartz crystal microbalance sensor. The DPPC-LUVs depositions were the major vesicular lipid bilayer on the surface, while the deposited egg PC-LUVs formed the planar lipid bilayer. The roles of phosphoglycerides and the cholesterol contents in the mechanism and the conformation of lipid vesicles onto the surface were discussed. (c) 2006 Elsevier B.V. All rights reserved.