Electrical surface potential of pulmonary surfactant

被引:28
|
作者
Leonenko, Zoya
Rodenstein, Mathias
Doehner, Jana
Eng, Lukas M.
Amrein, Matthias
机构
[1] Univ Calgary, Fac Med, Dept Cell Biol & Anat, Calgary, AB T2N 4N1, Canada
[2] Tech Univ Dresden, Inst Appl Photophys, D-01062 Dresden, Germany
关键词
AIR-WATER-INTERFACE; PROBE FORCE MICROSCOPY; POLYPEPTIDE SP-C; SCANNING FORCE; PROTEIN-B; ULTRAFINE PARTICLES; LIPID MONOLAYERS; LUNG SURFACTANT; DIPOLE-MOMENTS; CHOLESTEROL;
D O I
10.1021/la061718g
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Pulmonary surfactant is a mixed lipid protein substance of defined composition that self-assembles at the air-lung interface into a molecular film and thus reduces the interfacial tension to close to zero. A very low surface tension is required for maintaining the alveolar structure. The pulmonary surfactant film is also the first barrier for airborne particles entering the lung upon breathing. We explored by frequency modulation Kelvin probe force microscopy (FM-KPFM) the structure and local electrical surface potential of bovine lipid extract surfactant (BLES) films. BLES is a clinically used surfactant replacement and here served as a realistic model surfactant system. The films were distinguished by a pattern of molecular monolayer areas, separated by patches of lipid bilayer stacks. The stacks were at positive electrical potential with respect to the surrounding monolayer areas. We propose a particular molecular arrangement of the lipids and proteins in the film to explain the topographic and surface potential maps. We also discuss how this locally variable surface potential may influence the retention of charged or polar airborne particles in the lung.
引用
收藏
页码:10135 / 10139
页数:5
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