Sediment genotoxicity in the Tiete River (Sao Paulo, Brazil): In vitro comet assay versus in situ micronucleus assay studies

被引：67

作者：

Rocha, Paula Suares ^{[1
]}

Luvizotto, George Luiz ^{[2
]}

Kosmehl, Thomas ^{[1
]}

Boettcher, Melanie ^{[1
]}

Storch, Volker ^{[1
]}

Braunbeck, Thomas ^{[1
]}

Hollert, Henner ^{[3
]}

机构：

[1] Heidelberg Univ, Aquat Ecol & Toxicol Sect, Heidelberg Inst Zool, D-69120 Heidelberg, Germany

[2] Heidelberg Univ, Inst Mineral, D-69120 Heidelberg 10, Germany

[3] Rhein Westfal TH Aachen, Dept Ecosyst Anal, Inst Environm Res Biol 5, D-52074 Aachen, Germany

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2009年 / 72卷 / 07期

关键词：

Comet assay; Fish erythrocytes; Genotoxicity; Micronucleus assay; Sediments; RTL-W1 cell line; Tiete River; Brazil; Effluent; BARRA-BONITA-RESERVOIR; OREOCHROMIS-NILOTICUS; DNA-DAMAGE; RAINBOW-TROUT; WATER; TILAPIA; FISH; GILL; EUTROPHICATION; ERYTHROCYTES;

D O I：

10.1016/j.ecoenv.2009.04.013

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tiete River sediments (Sao Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from Sao Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tiete River warrants measures to reduce the input of toxic effluents. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1842 / 1848

页数：7

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