Carbon isotope fractionation in phospholipid fatty acid biomarkers of bacteria and fungi native to an acid mine drainage lake

被引:30
|
作者
Cowie, Benjamin R. [1 ]
Slater, Gregory F. [1 ]
Bernier, Luc [1 ]
Warren, Lesley A. [1 ]
机构
[1] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON L8S 4K1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
MICROBIAL COMMUNITIES; LIPID BIOMARKERS; BIOSYNTHESIS; GROWTH; THIOBACILLUS; DISCRIMINATION; ENDOSYMBIONTS; FERROOXIDANS; OXIDATION; RELEVANCE;
D O I
10.1016/j.orggeochem.2009.06.004
中图分类号
P3 [地球物理学]; P59 [地球化学];
学科分类号
0708 ; 070902 ;
摘要
This study identifies isotope signatures associated with autotrophic and heterotrophic microbial communities that may provide a means to determine carbon cycling relationships in situ for acid mine drainage (AMD) sites. Stable carbon isotope ratios (delta C-13) of carbon sources, bulk cells, and membrane phospholipids (PLFA) were measured for autotrophic and heterotrophic microbial enrichment cultures from a mine tailings impoundment in northern Ontario, Canada, and for pure strains of the sulfur oxidizing bacteria Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The autotrophic enrichments had indistinguishable PLFA distributions from the pure cultures, and the PLFA cyc-C-19:0 was determined to be a unique biomarker in this system for these sulfur oxidizing bacteria. The PLFA distributions produced by the heterotrophic enrichments were distinct from the autotrophic distributions and the C-18:2 PLFA was identified as a biomarker for these heterotrophic enrichments. Genetic analysis (16S, 18S rRNA) of the heterotrophic cultures indicated that these communities were primarily composed of Acremonium fungi. Stable carbon isotope analysis revealed that bulk cellular material in all autotrophic cultures was depleted in delta C-13 by 5.6-10.9 parts per thousand relative to their atmospheric CO2 derived carbon source, suggesting that inorganic carbon fixation in these cultures is carbon limited. Individual PLFA from these autotrophs were further depleted by 8.2-14.6 parts per thousand compared to the bulk cell delta C-13, which are among the largest biosynthetic isotope fractionation factors between bulk cell and PLFA reported in the literature. In contrast, the heterotrophic bulk cells were not significantly fractionated in delta C-13 relative to their carbon source and heterotrophic PLFA ranged from 3 parts per thousand enriched to 4 parts per thousand depleted relative to the isotopic composition of their total biomass. These distinct PLFA biomarkers and isotopic fractionations associated with autotrophic and heterotrophic activity in this laboratory study provide potential biomarkers for delineating autotrophic and heterotrophic carbon cycling in AMD environments. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:956 / 962
页数:7
相关论文
共 50 条
  • [41] Isotope geochemistry of drainage from an acid mine impaired watershed, Oakland, California
    Butler, Thomas W.
    APPLIED GEOCHEMISTRY, 2007, 22 (07) : 1416 - 1426
  • [42] STABLE ISOTOPE GEOCHEMISTRY OF ACID-MINE DRAINAGE - EXPERIMENTAL OXIDATION OF PYRITE
    TAYLOR, BE
    WHEELER, MC
    NORDSTROM, DK
    GEOCHIMICA ET COSMOCHIMICA ACTA, 1984, 48 (12) : 2669 - 2678
  • [43] PHOSPHOLIPID AND FATTY-ACID COMPOSITION OF METHANOL-UTILIZING BACTERIA
    GOLDBERG, I
    JENSEN, AP
    JOURNAL OF BACTERIOLOGY, 1977, 130 (01) : 535 - 537
  • [44] Phospholipid fatty acid composition of size fractionated indigenous soil bacteria
    Lindahl, V
    Frostegard, A
    Bakken, L
    Baath, E
    SOIL BIOLOGY & BIOCHEMISTRY, 1997, 29 (9-10): : 1565 - 1569
  • [45] Revisiting the coupling of fatty acid to phospholipid synthesis in bacteria with FapR regulation
    Machinandiarena, Federico
    Nakamatsu, Leandro
    Schujman, Gustavo E.
    de Mendoza, Diego
    Albanesi, Daniela
    MOLECULAR MICROBIOLOGY, 2020, 114 (04) : 653 - 663
  • [46] The use of phospholipid fatty acid analysis to measure impact of acid rock drainage on microbial communities in sediments
    Ben-David, EA
    Holden, PJ
    Stone, DJM
    Harch, BD
    Foster, LJ
    MICROBIAL ECOLOGY, 2004, 48 (03) : 300 - 315
  • [47] The Use of Phospholipid Fatty Acid Analysis to Measure Impact of Acid Rock Drainage on Microbial Communities in Sediments
    E.A. Ben-David
    P.J. Holden
    D.J.M. Stone
    B.D. Harch
    L.J. Foster
    Microbial Ecology, 2004, 48 : 300 - 315
  • [48] Phytoremediation potential of native wetland species in acid mine drainage (AMD) conditions
    Wilkes, Rebecca
    Bayline, Jennifer
    Kilgore, Jason
    Skylstad, Peter
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 250
  • [49] A combined CaO/electrochemical treatment of the acid mine drainage from the "Robule" Lake
    Orescanin, Visnja
    Kollar, Robert
    JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, 2012, 47 (08): : 1186 - 1191
  • [50] RESPONSE OF A NEW RESERVOIR, LAKE ANNA, TO NUTRIENT INPUT AND ACID MINE DRAINAGE
    ARMITAGE, BJ
    SIMMONS, GM
    JOURNAL OF PHYCOLOGY, 1973, 9 : 9 - 10