A class III WRKY transcription factor in sugarcane was involved in biotic and abiotic stress responses

被引：45

作者：

Wang, Dongjiao ^{[1
]}

Wang, Ling ^{[1
]}

Su, Weihua ^{[1
]}

Ren, Yongjuan ^{[1
]}

You, Chuihuai ^{[1
,2
]}

Zhang, Chang ^{[1
]}

Que, Youxiong ^{[1
,3
]}

Su, Yachun ^{[1
,3
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Agr, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou 350002, Fujian, Peoples R China

[2] Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China

[3] Fujian Agr & Forestry Univ, Coll Agr, Key Lab Genet Breeding & Multiple Utilizat Crops, Minist Educ, Fuzhou 350002, Fujian, Peoples R China

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

GENOME-WIDE IDENTIFICATION; FUNCTIONAL-ANALYSIS; EXPRESSION ANALYSIS; FACTOR FAMILY; GENE FAMILY; NEGATIVE REGULATORS; DEFENSE RESPONSES; BINDING PROTEIN; RICE; OVEREXPRESSION;

D O I：

10.1038/s41598-020-78007-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

WRKY transcription factors play significant roles in plant stress responses. In this study, a class III WRKY gene ScWRKY5, was successfully isolated from sugarcane variety ROC22. The ScWRKY5 was a nucleus protein with transcriptional activation activity. The ScWRKY5 gene was constitutively expressed in all the sugarcane tissues, with the highest expression level in the stem epidermis and the lowest in the root. After inoculation with Sporisorium scitamineum for 1 d, the expression level of ScWRKY5 was significantly increased in two smut-resistant varieties (YZ01-1413 and LC05-136), while it was decreased in three smut-susceptible varieties (ROC22, YZ03-103, and FN40). Besides, the expression level of ScWRKY5 was increased by the plant hormones salicylic acid (SA) and abscisic acid (ABA), as well as the abiotic factors polyethylene glycol (PEG) and sodium chloride (NaCl). Transient overexpression of the ScWRKY5 gene enhanced the resistance of Nicotiana benthamiana to the tobacco bacterial pathogen Ralstonia solanacearum, however the transiently overexpressed N. benthamiana was more sensitive to the tobacco fungal pathogen Fusarium solani var. coeruleum. These results provide a reference for further research on the resistance function of sugarcane WRKY genes.

引用

页数：15

共 79 条

[11]

Connolly Michael A, 2006, CSH Protoc, V2006, DOI 10.1101/pdb.prot4105

[12] A gateway cloning vector set for high-throughput functional analysis of genes in planta [J].