Molecular Resolution Imaging of Protein Molecules in Liquid Using Frequency Modulation Atomic Force Microscopy

被引：36

作者：

Yamada, Hirofumi ^{[1
]}

Kobayashi, Kei ^{[2
]}

Fukuma, Takeshi

Hirata, Yoshiki ^{[3
]}

Kajita, Teruyuki ^{[1
]}

Matsushige, Kazumi ^{[1
]}

机构：

[1] Kyoto Univ, Dept Elect Sci & Engn, Kyoto 6158510, Japan

[2] Kyoto Univ, Innovat Collaborat Ctr, Kyoto 6158520, Japan

[3] Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan

来源：

APPLIED PHYSICS EXPRESS | 2009年 / 2卷 / 09期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

BACTERIORHODOPSIN; GROEL;

D O I：

10.1143/APEX.2.095007

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We demonstrated molecular resolution imaging of biological samples such as bacteriorhodopsin protein molecules in purple membrane and isolated chaperonin (GroEL) protein molecules, both adsorbed on mica using frequency modulation atomic force microscope (FM-AFM) in liquid. We also showed that the frequency noise of FM-AFM in liquid can be greatly reduced by the reduction of the noise-equivalent deflection of an optical beam deflection sensor. (C) 2009 The Japan Society of Applied Physics

引用

页数：3

共 14 条

[1] FREQUENCY-MODULATION DETECTION USING HIGH-Q CANTILEVERS FOR ENHANCED FORCE MICROSCOPE SENSITIVITY [J].

ALBRECHT, TR ;

GRUTTER, P ;

HORNE, D ;

RUGAR, D .

JOURNAL OF APPLIED PHYSICS, 1991, 69 (02) :668-673

[2]

Durig U, 1997, J APPL PHYS, V82, P3641, DOI 10.1063/1.365726

[3] True molecular resolution in liquid by frequency-modulation atomic force microscopy [J].

Fukuma, T ;

Kobayashi, K ;

Matsushige, K ;

Yamada, H .

APPLIED PHYSICS LETTERS, 2005, 86 (19) :1-3

[4] Development of low noise cantilever deflection sensor for multienvironment frequency-modulation atomic force microscopy [J].

Fukuma, T ;

Kimura, M ;

Kobayashi, K ;

Matsushige, K ;

Yamada, H .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2005, 76 (05)

[5] Revealing molecular-level surface structure of amyloid fibrils in liquid by means of frequency modulation atomic force microscopy [J].

Fukuma, Takeshi ;

Mostaert, Anika S. ;

Serpell, Louise C. ;

Jarvis, Suzanne P. .

NANOTECHNOLOGY, 2008, 19 (38)

[6] Tapping-mode atomic force microscopy produces faithful high-resolution images of protein surfaces [J].

Möller, C ;

Allen, M ;

Elings, V ;

Engel, A ;

Müller, DJ .

BIOPHYSICAL JOURNAL, 1999, 77 (02) :1150-1158

[7]

Morita S., 1997, NONCONTACT ATOMIC FO

[8] Chaperonins GroEL and GroES: Views from atomic force microscopy [J].

Mou, JX ;

Sheng, ST ;

Ho, RY ;

Shao, ZF .

BIOPHYSICAL JOURNAL, 1996, 71 (04) :2213-2221

[9] FORCE-INDUCED CONFORMATIONAL CHANGE OF BACTERIORHODOPSIN [J].

MULLER, DJ ;

BULDT, G ;

ENGEL, A .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 249 (02) :239-243

[10] Higher harmonic atomic force microscopy: Imaging of biological membranes in liquid [J].

Preiner, Johannes ;

Tang, Jilin ;

Pastushenko, Vasilli ;

Hinterdorfer, Peter .

PHYSICAL REVIEW LETTERS, 2007, 99 (04)

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