Examination of Oral Squamous Cell Carcinoma and Precancerous Lesions Using Proximity Extension Assay and Salivary RNA Quantification

被引：9

作者：

Scholtz, Beata ^{[1
]}

Vo Minh, Doan ^{[2
,3
]}

Kiss, Csongor ^{[4
]}

Tar, Ildiko ^{[5
]}

Kumar, Ajneesh ^{[2
,3
,6
]}

Tozser, Jozsef ^{[3
]}

Csosz, Eva ^{[2
,3
]}

Marton, Ildiko ^{[7
]}

机构：

[1] Univ Debrecen, Dept Biochem & Mol Biol, Genom Med & Bioinformat Core Facil, Fac Med, H-4032 Debrecen, Hungary

[2] Univ Debrecen, Dept Biochem & Mol Biol, Biomarker Res Grp, Fac Med, H-4032 Debrecen, Hungary

[3] Univ Debrecen, Dept Biochem & Mol Biol, Prote Core Facil, Fac Med, H-4032 Debrecen, Hungary

[4] Univ Debrecen, Dept Pediat, Fac Med, H-4032 Debrecen, Hungary

[5] Univ Debrecen, Dept Oral Med, Fac Dent, H-4032 Debrecen, Hungary

[6] Univ Debrecen, Doctoral Sch Mol Cell & Immune Biol, Fac Med, H-4032 Debrecen, Hungary

[7] Univ Debrecen, Dept Restorat Dent, Fac Dent, H-4032 Debrecen, Hungary

来源：

BIOMEDICINES | 2020年 / 8卷 / 12期

基金：

匈牙利科学研究基金会;

关键词：

saliva; oral cancer; precancerous lesions; proximity extension assay; mRNA; EPITHELIAL-MESENCHYMAL TRANSITION; LICHEN-PLANUS; INTERLEUKIN-6; CANCER; LEUKOPLAKIA; EXPRESSION; PATHWAY; PLASMA; GENES; ROLES;

D O I：

10.3390/biomedicines8120610

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Saliva is an easy-to access body fluid with high diagnostic potential. The utilization of saliva for oral cancer diagnosis can be an attractive possibility. Besides the oral cancer, it is important to better understand the precancerous lesions such as oral lichen planus (OLP) and leukoplakia (OLK). In order to examine the changes of salivary proteins in controls, patients with oral cancer, and patients with precancerous conditions, proximity extension assay was utilized. Some proteins and functions were characteristic to the examined groups and can serve as a starting point for further biomarker studies. The different nature of OLK and OLP was demonstrated, showing the malignant transformation and the inflammation as the prominent biological processes in the OLK and OLP, respectively. The salivary level of IL6 was verified using quantitative ELISA and the mRNA level was also studied. Elevated IL6 levels could be detected in precancerous groups compared to controls.

引用

页码：1 / 16

页数：16

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[21] Circulating interleukin 6 levels, blood pressure, and insulin sensitivity in apparently healthy men and women [J].