The epoxy fatty acid pathway enhances cAMP in mammalian cells through multiple mechanisms

被引：0
作者：
Matsumoto, Naoki [1 ,2 ]
Singh, Nalin [1 ,2 ]
Lee, Kin Sing [3 ]
Barnych, Bogdan [1 ,2 ]
Morisseau, Christophe [1 ,2 ]
Hammock, Bruce D. [1 ,2 ]
机构：
[1] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA
[2] Univ Calif Davis, Comprehens Canc Ctr, Davis, CA USA
[3] Michigan State Univ, Dept Pharmacol & Toxicol, E Lansing, MI USA
来源：
PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2022年 / 162卷
关键词：
Eicosanoid; Epoxyeicosatrienoicacid; Dihydroxyeicosatrienoicacid; Solubleepoxidehydrolase; CyclicAMP; EP2; Inflammation; RAM SEMINAL-VESICLES; SMOOTH-MUSCLE; 11,12-EPOXYEICOSATRIENOIC ACID; 14,15-EPOXYEICOSATRIENOIC ACID; PHARMACOLOGICAL INHIBITION; DOCOSAHEXAENOIC ACID; HYDROLASE DEFICIENCY; INDUCED LIPOKINE; TUMOR-GROWTH; HUMAN PLASMA;
D O I：
10.1016/j.prostaglins.2022.106662
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cellular mechanism by which epoxy fatty acids (EpFA) improves disease status is not well characterized. Previous studies suggest the involvement of cellular receptors and cyclic AMP (cAMP). Herein, the action of EpFAs derived from linoleic acid (LA), arachidonic acid (ARA), and docosahexaenoic acid on cAMP levels was studied in multiple cell types to elucidate relationships between EpFAs, receptors and cells' origin. cAMP levels were enhanced in HEK293 and LLC-PK1 cells by EpFAs from LA and ARA. Using selective antagonists, the EpFA effects on cAMP levels appear dependent on the prostaglandin E2 receptor 2 (EP2) but not 4 (EP4). Human coronary artery smooth muscle cells responded similarly to the EpFAs. However, we were not able to show the involvement of any of the receptors tested in this cell type. The results pinpointed distinct cell lines and receptor subtypes that natively respond to EpFA.
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页数：8
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共 60 条
  • [1] Regulation of forskolin-induced cAMP production by cytochrome P450 epoxygenase metabolites of arachidonic acid in HEK293 cells
    Abukhashim, Mohamed
    Wiebe, Glenis J.
    Seubert, John M.
    [J]. CELL BIOLOGY AND TOXICOLOGY, 2011, 27 (05) : 321 - 332
  • [2] Cytochrome P450 derived epoxidized fatty acids as a therapeutic tool against neuroinflammatory diseases
    Atone, Jogen
    Wagner, Karen
    Hashimoto, Kenji
    Hammock, Bruce D.
    [J]. PROSTAGLANDINS & OTHER LIPID MEDIATORS, 2020, 147
  • [3] Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis
    Atwood B.K.
    Lopez J.
    Wager-Miller J.
    Mackie K.
    Straiker A.
    [J]. BMC Genomics, 12 (1)
  • [4] Universal Real-Time PCR-Based Assay for Lentiviral Titration
    Barczak, Wojciech
    Suchorska, Wiktoria
    Rubis, Blazej
    Kulcenty, Katarzyna
    [J]. MOLECULAR BIOTECHNOLOGY, 2015, 57 (02) : 195 - 200
  • [5] Soluble Epoxide Hydrolase Deficiency or Inhibition Attenuates Diet-induced Endoplasmic Reticulum Stress in Liver and Adipose Tissue
    Bettaieb, Ahmed
    Nagata, Naoto
    AbouBechara, Daniel
    Chahed, Samah
    Morisseau, Christophe
    Hammock, Bruce D.
    Haj, Fawaz G.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (20) : 14189 - 14199
  • [6] CARROLL MA, 1993, J BIOL CHEM, V268, P12260
  • [7] Adenosine2A receptor vasodilation of rat preglomerular microvessels is mediated by EETs that activate the cAMP/PKA pathway
    Carroll, Mairead A.
    Doumad, Anabel B.
    Li, Jing
    Cheng, Monica K.
    Falck, J. R.
    McGiff, John C.
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2006, 291 (01) : F155 - F161
  • [8] Epoxide metabolites of arachidonate and docosahexaenoate function conversely in acute kidney injury involved in GSK3β signaling
    Deng, Bing-Qing
    Luo, Ying
    Kang, Xin
    Li, Chang-Bin
    Morisseau, Christophe
    Yang, Jun
    Lee, Kin Sing Stephen
    Huang, Jian
    Hu, Da-Yong
    Wu, Ming-Yu
    Peng, Ai
    Hammock, Bruce D.
    Liu, Jun-Yan
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (47) : 12608 - 12613
  • [9] The Biological Actions of 11,12-Epoxyeicosatrienoic Acid in Endothelial Cells Are Specific to the R/S-Enantiomer and Require the Gs Protein
    Ding, Yindi
    Froemel, Timo
    Popp, Ruediger
    Falck, John R.
    Schunck, Wolf-Hagen
    Fleming, Ingrid
    [J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2014, 350 (01) : 14 - 21
  • [10] Prostaglandin E2 promotes integrin αVβ3-dependent endothelial cell adhesion, Rac-activation, and spreading through cAMP/PKA-dependent signaling
    Dormond, O
    Bezzi, M
    Mariotti, A
    Rüegg, C
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (48) : 45838 - 45846
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