Fluorescence incoherent color holography

被引:114
作者
Rosen, Joseph
Brooker, Gary
机构
[1] Johns Hopkins Univ, Dept Biol, Integrated Imaging Ctr, Rockville, MD 20850 USA
[2] Ben Gurion Univ Negev, Dept Elect & Comp Engn, IL-84105 Beer Sheva, Israel
来源
OPTICS EXPRESS | 2007年 / 15卷 / 05期
关键词
D O I
10.1364/OE.15.002244
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We present a new imaging method to record multicolor digital holograms from objects emitting fluorescent light. The fluorescent light specific to the emission wavelength of various fluorescent dyes after excitation of three dimensional (3D) objects is recorded on a digital monochrome camera after reflection from a diffractive optical element ( DOE). For each wavelength of fluorescent emission, the camera sequentially records three holograms reflected from the DOE, each with a different phase factor of the DOE's function. The three holograms are superposed in a computer to create a complex valued Fresnel hologram of each fluorescent emission. The holograms for each fluorescent color are further combined in a computer to produce a multicolored fluorescence hologram and 3D color image. (c) 2007 Optical Society of America.
引用
收藏
页码:2244 / 2250
页数:7
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