Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network

被引：30

作者：

Garza-Gonzalez, Elvira ^{[1
]}

Bocanegra-Ibarias, Paola ^{[1
]}

Bobadilla-del-Valle, Miriam ^{[2
]}

Alfredo Ponce-de-Leon-Garduno, Luis ^{[2
]}

Esteban-Kenel, Veronica ^{[2
]}

Silva-Sanchez, Jesus ^{[3
]}

Garza-Ramos, Ulises ^{[3
]}

Barrios-Camacho, Humberto ^{[3
]}

Esau Lopez-Jacome, Luis ^{[4
]}

Colin-Castro, Claudia A. ^{[4
]}

Franco-Cendejas, Rafael ^{[4
]}

Flores-Trevino, Samantha ^{[1
]}

Morfin-Otero, Rayo ^{[5
,6
]}

Rojas-Larios, Fabian ^{[7
]}

Pablo Mena-Ramirez, Juan ^{[8
]}

Guadalupe Fong-Camargo, Maria ^{[9
]}

Teresita Morales-De-la-Pena, Cecilia ^{[10
]}

Garcia-Mendoza, Lourdes ^{[11
]}

Victoria Choy-Chang, Elena ^{[12
]}

Karina Aviles-Benitez, Laura ^{[13
]}

Manuel Feliciano-Guzman, Jose ^{[14
]}

Lopez-Gutierrez, Eduardo ^{[15
]}

Gil-Veloz, Mariana ^{[16
]}

Manuel Barajas-Magallon, Juan ^{[17
]}

Aguirre-Burciaga, Efren ^{[18
]}

Isabel Lopez-Moreno, Laura ^{[19
]}

Thelma Martinez-Villarreal, Rebeca ^{[20
]}

Luis Canizales-Oviedo, Jorge ^{[21
]}

Miguel Cetina-Umana, Carlos ^{[22
]}

Romero-Romero, Daniel ^{[23
]}

David Bello-Pazos, Fidencio ^{[24
]}

Eric Barlandas-Rendon, Nicolas Rogelio ^{[25
]}

Yanelli Maldonado-Anicacio, Joyarib ^{[26
]}

Bolado-Martinez, Enrique ^{[27
]}

Galindo-Mendez, Mario ^{[28
]}

Perez-Vicelis, Talia ^{[29
]}

Alavez-Ramirez, Norma ^{[29
]}

Mendez-Sotelo, Braulio J. ^{[30
]}

Francisco Cabriales-Zavala, Juan ^{[31
]}

Citlali Nava-Pacheco, Yirla ^{[32
]}

Irene Moreno-Mendez, Martha ^{[33
]}

Garcia-Romo, Ricardo ^{[34
]}

Rafael Silva-Gamino, Aldo ^{[35
]}

Maria Avalos-Aguilera, Ana ^{[36
]}

Asuncion Santiago-Calderon, Maria ^{[37
]}

Lopez-Garcia, Maribel ^{[38
]}

del Consuelo Velazquez-Acosta, Maria ^{[39
]}

Isabel Cobos-Canul, Dulce ^{[40
]}

Del Rosario Vazquez-Larios, Maria ^{[41
]}

Elizabeth Ortiz-Porcayo, Ana ^{[42
]}

机构：

[1] Univ Autonoma Nuevo Leon, Hosp Univ Dr Jose E Gonzalez, Monterrey, Nuevo Leon, Mexico

[2] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Ciudad De Mexico, Mexico

[3] Inst Nacl Salud Publ, Cuernavaca, Morelos, Mexico

[4] Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Ciudad De Mexico, Mexico

[5] Hosp Civil Guadalajara, Guadalajara, Jalisco, Mexico

[6] Inst Patol Infecciosa, Guadalajara, Jalisco, Mexico

[7] Hosp Reg Univ Colima, Colima, Colima, Mexico

[8] Univ Guadalajara, Ctr Univ Altos CUALTOS, Hosp Gen Zona Tepatitlan Morelos 21, Tepatitlan De Morelos, Jalisco, Mexico

[9] Hosp Gen Reg 1, Chihuahua, Chihuahua, Mexico

[10] Hosp Gen Especialidades Juan Maria de Salvatierra, La Paz, Baja California, Mexico

[11] Hosp Angeles Valle Oriente, Monterrey, Nuevo Leon, Mexico

[12] Hosp Gen Zona 1, Tapachula, Chiapas, Mexico

[13] Hosp Infantil Morelia, Morelia, Michoacan, Mexico

[14] Hosp Especialidades Pediat Chiapas, Tuxtla Gutierrez, Chiapas, Mexico

[15] Hosp Reg Alta Especialidad Oaxaca, San Bartolo Coyotepec, Oaxaca, Mexico

[16] Hosp Reg Alta Especialidad Bajio, Guanajuato, Guanajuato, Mexico

[17] Lab Dipromi, Morelia, Michoacan, Mexico

[18] Hosp Reg Delicias, Delicias, Chihuahua, Mexico

[19] Galenia Hosp, Cancun, Quintana Roo, Mexico

[20] Univ Autonoma Nuevo Leon, Ctr Univ Salud, Lab Vicente Guerrero, Monterrey, Nuevo Leon, Mexico

[21] Univ Autonoma Nuevo Leon, Ctr Univ Salud, Lab Pueblo Nuevo, Monterrey, Nuevo Leon, Mexico

[22] Hosp Materno Infantil Morelos, Chetmal, Quintana Roo, Mexico

[23] Lab Anal Bioquim Clin Louis Pasteur Toluca, Toluca, Mexico

[24] Hosp H Queretaro, Queretaro, Queretaro, Mexico

[25] Lab Bioclin, Chilpancingo, Guerrero, Mexico

[26] Hosp Gen Chilpancingo, Chilpancingo, Guerrero, Mexico

[27] Univ Sonora, Hermosillo, Sonora, Mexico

[28] Labs Galindo SC, Oaxaca, Oaxaca, Mexico

[29] Hosp Reg Bicentenario Independencia ISSSTE, Tultitlan, Estado De Mexic, Mexico

[30] Hosp Gen Dr Manuel Gea Gonzalez, Ciudad De Mexico, Mexico

[31] Swiss Hosp, Monterrey, Nuevo Leon, Mexico

[32] Hosp Nino Poblano, Puebla, Mexico

[33] Labs Ctr, Zamnora, Michoacan, Mexico

[34] Centenario Hosp Miguel Hidalgo, Aguascalientes, Aguascalientes, Mexico

[35] Hosp Angeles Morelia, Morelia, Michoacan, Mexico

[36] Hosp Gen Dr Miguel Silva, Morelia, Michoacan, Mexico

[37] Hosp Gen Zona 1 Dr Demetrio Mayoral Pardo, Oaxaca, Oaxaca, Mexico

[38] Hosp Madre & Nino Guerrerense, Chilpancingo, Guerrero, Mexico

[39] Inst Nacl Cancerol, Ciudad De Mexico, Mexico

[40] Hosp Gen Chetumal, Chetmal, Quintana Roo, Mexico

[41] Inst Nacl Cardiol Ignacio Chavez, Ciudad De Mexico, Mexico

[42] Hosp Reg Monterrey ISSSTE, Monterrey, Nuevo Leon, Mexico

[43] Hosp Mil Reg Especialidades, Mazatlan, Sinaloa, Mexico

[44] Ctr Diagnost Microbiol SA CV, Morelia, Michoacan, Mexico

[45] Hosp Especialidades Pediat Leon, Guanajuato, Mexico

[46] Hosp Ginequito, Bioclinsa, Monterrey, Nuevo Leon, Mexico

来源：

PLOS ONE | 2021年 / 16卷 / 03期

关键词：

METALLO-BETA-LACTAMASE; TERTIARY CARE CENTER; KLEBSIELLA-PNEUMONIAE; ACINETOBACTER-BAUMANNII; ESCHERICHIA-COLI; RAPID DETECTION; MULTIPLEX PCR; CARBAPENEMASES; GENES; ENTEROBACTERIACEAE;

D O I：

10.1371/journal.pone.0248614

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum beta-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. Results Among blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, bla(TEM), bla(SHV), and bla(CTX) were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, bla(TEM), bla(SHV), and bla(CTX) were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was bla(CTX-M-15). Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was bla(NDM-1) (81.5%), followed by bla(OXA-232) (14.8%) and bla(oxa-181)(7.4%), in A. baumannii was bla(OXA-24) (76%) and in P. aeruginosa, was bla(IMP) (25.3%), followed by bla(GES) and bla(VIM) (13.1% each). Conclusion Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL bla(CTX-M-15) exists in both E. coli and K. pneumoniae.

引用

页数：16

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