Volta phase plate cryo-EM of the small protein complex Prx3

被引：49

作者：

Khoshouei, Maryam ^{[1
]}

Radjainia, Mazdak ^{[2
,3
]}

Phillips, Amy J. ^{[4
]}

Gerrard, Juliet A. ^{[2
,5
,6
]}

Mitra, Alok K. ^{[2
]}

Plitzko, Juergen M. ^{[1
]}

Baumeister, Wolfgang ^{[1
]}

Danev, Radostin ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany

[2] Univ Auckland, Sch Biol Sci, Auckland 1042, New Zealand

[3] Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic 3800, Australia

[4] Lincoln Agritech Ltd, Lincoln 7640, NE, New Zealand

[5] Victoria Univ, MacDiarmid Inst Adv Mat & Nanotechnol, Wellington 6140, New Zealand

[6] Univ Auckland, Sch Chem Sci, 23 Symonds St, Auckland 1010, New Zealand

来源：

NATURE COMMUNICATIONS | 2016年 / 7卷

关键词：

CRYOELECTRON MICROSCOPY; ELECTRON-MICROSCOPY; ANGSTROM RESOLUTION; PEROXIREDOXIN; CRYOMICROSCOPY; TOMOGRAPHY;

D O I：

10.1038/ncomms10534

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 angstrom resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.

引用

页数：5

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