Specificity Determination in Saccharomyces cerevisiae Killer Virus Systems

被引：9

作者：

Aitmanaite, Lina ^{[1
]}

Konovalovas, Aleksandras ^{[1
]}

Medvedevas, Povilas ^{[1
]}

Serviene, Elena ^{[2
]}

Serva, Saulius ^{[1
]}

机构：

[1] Vilnius Univ, Life Sci Ctr, Inst Biosci, Lab Nucle Acid Biochem, LT-10257 Vilnius, Lithuania

[2] Nat Res Ctr, Lab Genet, LT-08412 Vilnius, Lithuania

来源：

MICROORGANISMS | 2021年 / 9卷 / 02期

关键词：

Saccharomyces yeast; Totiviridae; dsRNA; LA virus; killer virus;

D O I：

10.3390/microorganisms9020236

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Saccharomyces yeasts are widely distributed in the environment and microbiota of higher organisms. The killer phenotype of yeast, encoded by double-stranded RNA (dsRNA) virus systems, is a valuable trait for host survival. The mutual relationship between the different yet clearly defined LA and M virus pairs suggests complex fitting context. To define the basis of this compatibility, we established a system devoted to challenging inherent yeast viruses using viral proteins expressed in trans. Virus exclusion by abridged capsid proteins was found to be complete and nonspecific, indicating the presence of generic mechanisms of Totiviridae maintenance in yeast cells. Indications of specificity in both the exclusion of LA viruses and the maintenance of M viruses by viral capsid proteins expressed in trans were observed. This precise specificity was further established by demonstrating the importance of the satellite virus in the maintenance of LA virus, suggesting the selfish behavior of M dsRNA.

引用

页码：1 / 15

页数：14

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