Relationship Between Seed Quality and Changes in Conjugated Polyamine in Plasma Membrane Purified from Wheat Embryos during Grain Ripening

被引:0
|
作者
Du, H. Y. [1 ,2 ]
Zhou, X. G. [3 ]
Liu, G. T. [2 ]
Liu, H. P. [1 ]
Kurtenbach, R. [1 ]
机构
[1] Zhoukou Normal Univ, Key Lab Plant Genet & Mol Breeding, Coll Life Sci & Agron, Zhoukou 466001, Henan, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China
[3] Chinese Acad Agr Sci, Farmland Irrigat Res Inst, Xinxiang 453003, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
Triticum aestivum; conjugated polyamines; plasma membrane; grain ripening; wheat embryos; OSMOTIC-STRESS; PUTRESCINE; ETHYLENE; SPIKELETS; SUPERIOR; INFERIOR; CULTURES; GROWTH;
D O I
10.1134/S1021443717030074
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In order to explore the relationship between seed quality and changes in conjugated polyamine in plasma membrane purified from wheat embryos during grain ripening. Plasma membrane (PM) vesicles were isolated from the embryos of ripening wheat (Triticum aestivum L.) grains by the gradient centrifugation method. The contents of polyamines conjugated (covalently and non-covalently) to the PM vesicles were investigated. Results showed that after pollination, from the 22nd to the 32nd day, the embryos of wheat grains underwent dehydration, as judged by the decrease of embryo relative water content (ERWC). During embryo ripening, non-covalently conjugated (NCC)-Spd and NCC-Spm, covalently conjugated (CC)-Put and CC-Spd contents increased markedly, while relative embryo cell vigor (RECV) decreased slightly. The treatment with methylglyoxyl-bis (guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), inhibited the increases of the NCC-Spd and NCC-Spm contents, enhanced the decrease of RECV simultaneously, and decreased mature seed relative germination potential (RGP). The effects of MGBG were reversed by exogenous Spd and Spm. Phenanthrolin (o(-)Phen), an inhibitor of transglutaminase (TGase), inhibited the increases of CC-Put and CC-Spd contents, enhanced the decrease of RECV simultaneously, and decreased mature seed RGP. These results suggest that during embryo ripening, the levels of NCC-Spd, NCC-Spm, CC-Put, and CC-Spd increase, and these increases might affect embryo cell vigor and seed germination potential.
引用
收藏
页码:325 / 332
页数:8
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