The TEL gene products: Nuclear phosphoproteins with DNA binding properties

被引：96
作者：
Poirel, H
Oury, C
Carron, C
Duprez, E
Laabi, Y
Tsapis, A
Romana, SP
Mauchauffe, M
LeConiat, M
Berger, R
Ghysdael, J
Bernard, OA
机构：
[1] INST GENET MOL,INSERM,U301,F-75010 PARIS,FRANCE
[2] INST GENET MOL,SD 401 301 CNRS,F-75010 PARIS,FRANCE
[3] CTR UNIV ORSAY,INST CURIE,SECT RECH,F-91405 ORSAY,FRANCE
来源：
ONCOGENE | 1997年 / 14卷 / 03期
关键词：
TEL; ETS; translocation; transcription factor; leukemia;
D O I：
10.1038/sj.onc.1200829
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human TEL gene is involved in several 12p13 chromosomal abnormalities present in various human hematological malignancies, the most frequent being the t(12;21)(p13;q22), specific for childhood acute lymphoblastic leukemia, The predicted product of TEL harbours an amino acid region similar to the ETS DNA binding domain. We now report the isolation of the murine TEL cDNA and the characterization of the human TEL proteins. Human and murine TEL proteins are particularly homologous within their aminoterminal regions and their ETS domains. TEL proteins are nuclear and display specific DNA binding activity toward classical ETS binding sites. In addition, we show that TEL mRNAs initiate translation at either of the two first in-frame ATGs (codon 1 and 43) to encode 50 kDa and 57 kDa TEL proteins. In vivo, each of these primary translational products is modified by multiple phosphorylation events.
引用
收藏
页码：349 / 357
页数：9
相关论文
共 45 条
[1]   DNA-BINDING AND TRANSCRIPTIONAL ACTIVATION PROPERTIES OF THE EWS-FLI-1 FUSION PROTEIN RESULTING FROM THE T(1122) TRANSLOCATION IN EWING SARCOMA [J].
BAILLY, RA ;
BOSSELUT, R ;
ZUCMAN, J ;
CORMIER, F ;
DELATTRE, O ;
ROUSSEL, M ;
THOMAS, G ;
GHYSDAEL, J .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) :3230-3241
[2]  
BERNARD OA, 1996, IN PRESS LEUKEMIA LY
[3]   A SINGLE AMINO-ACID SUBSTITUTION IN THE ETS DOMAIN ALTERS CORE DNA-BINDING SPECIFICITY OF ETS1 TO THAT OF THE RELATED TRANSCRIPTION FACTORS ELF1 AND E74 [J].
BOSSELUT, R ;
LEVIN, J ;
ADJADJ, E ;
GHYSDAEL, J .
NUCLEIC ACIDS RESEARCH, 1993, 21 (22) :5184-5191
[4]   THE PRODUCT OF THE C-ETS-1 PROTOONCOGENE AND THE RELATED ETS2 PROTEIN ACT AS TRANSCRIPTIONAL ACTIVATORS OF THE LONG TERMINAL REPEAT OF HUMAN T-CELL LEUKEMIA-VIRUS HTLV-1 [J].
BOSSELUT, R ;
DUVALL, JF ;
GEGONNE, A ;
BAILLY, M ;
HEMAR, A ;
BRADY, J ;
GHYSDAEL, J .
EMBO JOURNAL, 1990, 9 (10) :3137-3144
[5]   DEFINITION OF AN ETS1 PROTEIN DOMAIN REQUIRED FOR NUCLEAR-LOCALIZATION IN CELLS AND DNA-BINDING ACTIVITY INVITRO [J].
BOULUKOS, KE ;
POGNONEC, P ;
RABAULT, B ;
BEGUE, A ;
GHYSDAEL, J .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (12) :5718-5721
[6]   THE ETS DOMAIN PROTEIN POINTED-P2 IS A TARGET OF MAP KINASE IN THE SEVENLESS SIGNAL-TRANSDUCTION PATHWAY [J].
BRUNNER, D ;
DUCKER, K ;
OELLERS, N ;
HAFEN, E ;
SCHOLZ, H ;
KLAMBT, C .
NATURE, 1994, 370 (6488) :386-389
[7]  
BUIJS A, 1995, ONCOGENE, V10, P1511
[8]   LOSS OF HETEROZYGOSITY IN THE CHROMOSOMAL REGION 12P12-13 IS VERY COMMON IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA AND PERMITS THE PRECISE LOCALIZATION OF A TUMOR-SUPPRESSOR GENE DISTINCT FROM P7(KIP1) [J].
CAVE, H ;
GERARD, B ;
MARTIN, E ;
GUIDAL, C ;
DEVAUX, I ;
WEISSENBACH, J ;
ELION, J ;
VILMER, E ;
GRANDCHAMP, B .
BLOOD, 1995, 86 (10) :3869-3875
[9]   GENE FUSION WITH AN ETS DNA-BINDING DOMAIN CAUSED BY CHROMOSOME-TRANSLOCATION IN HUMAN TUMORS [J].
DELATTRE, O ;
ZUCMAN, J ;
PLOUGASTEL, B ;
DESMAZE, C ;
MELOT, T ;
PETER, M ;
KOVAR, H ;
JOUBERT, I ;
DEJONG, P ;
ROULEAU, G ;
AURIAS, A ;
THOMAS, G .
NATURE, 1992, 359 (6391) :162-165
[10]  
GHYSDAEL J, 1995, PATHOL BIOL, V43, P202
12345