Survey for Toxoplasma gondii by PCR detection in meat for human consumption in Colombia

被引：31
作者：
Franco-Hernandez, Erika N. [1 ]
Acosta, Alejandro [2 ]
Cortes-Vecino, Jesus [1 ]
Enrique Gomez-Marin, Jorge [2 ]
机构：
[1] Univ Nacl Colombia, Fac Med Vet & Zootecn, Lab Parasitol Vet, Bogota, Colombia
[2] Univ Quindio, Fac Ciencias Salud, Ctr Invest Biomed, Grp Parasitol Mol GEPAMOL, Ave Bolivar 12N, Armenia, Quindio, Colombia
来源：
PARASITOLOGY RESEARCH | 2016年 / 115卷 / 02期
关键词：
Toxoplasma; Meat; Colombia; PCR; B1; sequence; PREVALENCE; INFECTION; SAMPLES; BRAZIL;
D O I：
10.1007/s00436-015-4790-7
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The overall risk for toxoplasmosis in meat produced in Colombia is unknown. We analyzed by PCR assay meat samples for human consumption in two types of plants in Colombia: 120 samples from class I plants (60 samples from chicken, 30 from swine and 30 from beef) and 60 from class II plants (30 samples from beef and 30 from swine). Presence of Toxoplasma DNA was established by targeted B1 nested PCR assay. We detected 79 (43 %) samples that were positive by B1 nested PCR (33 from chicken, 22 from beef, and 24 from pork). No differences were found by region or species. Eleven positive samples were confirmed by sequencing of the B1 repeated region. Some polymorphisms were detected without relation with clonal groups nor meat species. Food animals are highly exposed to Toxoplasma in Colombia. Detailed studies are needed to establish the reasons for differences in Toxoplasma prevalence between farms, regarding practices of animal food production.
引用
收藏
页码：691 / 695
页数：5
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