Investigation of Hydrolysis Using Cellulase Enzyme Produced From Cow Rumen And Fermentation Method for Producing Ethanol from Nypa (Nypa fruticans Wurmb) Midrib

Nypa (Nypa fruticans Wurmb) has considered to have high potential for producing ethanol. This research was mainly focus on investigation to find: (1) influence of enzyme concentration and hydrolysis duration in producing reducing sugar from nypa midrib and (2) influence of Saccharomyces cerevisiae concentration and duration of fermentation process to convert the nypa midrib reducing sugar for producing ethanol. Experiments consisted of hydrolysis and fermentation processes. In hydrolysis process, two factors were tested, i. e. concentration of cellulase enzyme (5 ml, 10 ml, and 25 ml) and duration of hydrolysis process (12 hour, 24 hours, 36 hours, 48, hours, and 60 hours). Three replications were applied for each treatment. The best result of the hydrolysis was applied in fermentation experiments. Two factors, i. e. concentration of Saccharomyces cerevisiae (12.5% (v/v), 25% (v/v), and 37.5% (v/v)) and fermentation duration (4 days, 6 days, 8 days). Experiment results indicated that application of 5 ml of enzyme concentration and 12 hours duration of hydrolysis gave highest production of reducing sugar in hydrolysis process. Highest production of ethanol was gained by application of 37.5% concentration of S. cerevisae and 144 hours of fermentation process.