An improved protocol for ICP-MS-based assessment of the cellular uptake of metal-based nanoparticles

被引:12
作者
Kruszewska, Joanna [1 ]
Kur, Agnieszka [1 ]
Kulpinska, Dominika [2 ]
Grabowska-Jadach, Ilona [2 ]
Matczuk, Magdalena [1 ]
Keppler, Bernhard K. [3 ]
Timerbaev, Andrei R. [4 ]
Jarosz, Maciej [1 ]
机构
[1] Warsaw Univ Technol, Fac Chem, Chair Analyt Chem, Noakowskiego St 3, PL-00664 Warsaw, Poland
[2] Warsaw Univ Technol, Fac Chem, Chair Med Biotechnol, Noakowskiego St 3, PL-00664 Warsaw, Poland
[3] Univ Vienna, Inst Inorgan Chem, Waehringer Str 42, A-1090 Vienna, Austria
[4] Vernadsky Inst Geochem & Analyt Chem, Kosygin St 19, Moscow 119991, Russia
关键词
Gold: nanoparticles; Cancer cells; ICP-MS; Cellular uptake; Serum-protein corona; GOLD NANOPARTICLES; PENETRATION; CELLS;
D O I
10.1016/j.jpba.2019.06.006
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Along with a growing interest in biomedical applications of metal-based nanoparticles, there is a compelling need in systematic information on their behavior in human body systems, preferably at the cellular level. However, in most of the in-vitro uptake experiments, the nanomaterial was applied in its native form that in reality can hardly reach the cell. In this work, we developed an improved procedure in which prior to addition to the cells the particles are converted into the protein conjugates by incubation in human serum. The procedure was tested for gold nanoparticles of different size, chosen as a representative nanomaterial on multifunctional medicinal use, and MCF-7 cell line. Using ICP-MS to measure intracellular metal concentration, it was shown that an original state has significant effect on particle internalization. The protein corona significantly inhibits the uptake amount by MCF-7 cells, with the greatest influence (a 15-fold decrease compared to uncoated particles) being exerted over the smallest, 5-nm particles (3 pg Au/cell). Conjugates of larger particles (20 and 50 nm) are taken up more effectively (45 and 34 pg Au/cell, respectively). The advanced protocol makes the uptake results more reliable and its implementation may accelerate the preclinical development of metal-based nanoparticles as a viable theranostic implement. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:300 / 304
页数:5
相关论文
共 21 条
[1]   Could nanoparticle corona characterization help for biological consequence prediction? [J].
Brun E. ;
Sicard – Roselli C. .
Cancer Nanotechnology, 2014, 5 (1)
[2]   Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner [J].
Cheng, Xiaju ;
Tian, Xin ;
Wu, Anqing ;
Li, Jianxiang ;
Tian, Jian ;
Chong, Yu ;
Chai, Zhifang ;
Zhao, Yuliang ;
Chen, Chunying ;
Ge, Cuicui .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (37) :20568-20575
[3]   Mechanics of receptor-mediated endocytosis [J].
Gao, HJ ;
Shi, WD ;
Freund, LB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (27) :9469-9474
[4]   Size-Dependent Localization and Penetration of Ultrasmall Gold Nanoparticles in Cancer Cells, Multicellular Spheroids, and Tumors in Vivo [J].
Huang, Keyang ;
Ma, Huili ;
Liu, Juan ;
Huo, Shuaidong ;
Kumar, Anil ;
Wei, Tuo ;
Zhang, Xu ;
Jin, Shubin ;
Gan, Yaling ;
Wang, Paul C. ;
He, Shengtai ;
Zhang, Xiaoning ;
Liang, Xing-Jie .
ACS NANO, 2012, 6 (05) :4483-4493
[5]   Methodologies and approaches for the analysis of cell-nanoparticle interactions [J].
Ivask, Angela ;
Mitchell, Andrew J. ;
Malysheva, Anzhela ;
Voelcker, Nicolas H. ;
Lombi, Enzo .
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY, 2018, 10 (03)
[6]   Nanoparticle-blood interactions: the implications on solid tumour targeting [J].
Lazarovits, James ;
Chen, Yih Yang ;
Sykes, Edward A. ;
Chan, Warren C. W. .
CHEMICAL COMMUNICATIONS, 2015, 51 (14) :2756-2767
[7]   Cellular processing of gold nanoparticles: CE-ICP-MS evidence for the speciation changes in human cytosol [J].
Legat, Joanna ;
Matczuk, Magdalena ;
Timerbaev, Andrei R. ;
Jarosz, Maciej .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2018, 410 (03) :1151-1156
[8]   Mechanisms of cell uptake, inflammatory potential and protein corona effects with gold nanoparticles [J].
Li, Yang ;
Monteiro-Riviere, Nancy A. .
NANOMEDICINE, 2016, 11 (24) :3185-3203
[9]   Inorganic nanomaterials for bioimaging, targeted drug delivery and therapeutics [J].
Liang, Ruizheng ;
Wei, Min ;
Evans, David G. ;
Duan, Xue .
CHEMICAL COMMUNICATIONS, 2014, 50 (91) :14071-14081
[10]   Analytical methodology for studying cellular uptake, processing and localization of gold nanoparticles [J].
Matczuk, Magdalena ;
Ruzik, Lena ;
Aleksenko, Svetlana S. ;
Keppler, Bernhard K. ;
Jarosz, Maciej ;
Timerbaev, Andrei R. .
ANALYTICA CHIMICA ACTA, 2019, 1052 :1-9