Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants

被引：41

作者：

Lewsey, Mathew G. ^{[1
]}

Carr, John P. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

来源：

JOURNAL OF GENERAL VIROLOGY | 2009年 / 90卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

DEPENDENT RNA-POLYMERASE; ANTIVIRAL DEFENSE; INDUCED RESISTANCE; ARABIDOPSIS; INTERFERES; MOVEMENT; REPLICATION; MECHANISMS; SUPPRESSOR; INDUCTION;

D O I：

10.1099/vir.0.014555-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Salicylic acid (SA)-mediated resistance and RNA silencing are both important plant antiviral defence mechanisms. To investigate overlap between these resistance phenomena, we examined the ability of mutant Arabidopsis thaliana plants lacking DICER-like (DCL) encloribonucleases 2, 3 and 4 to exhibit SA-induced defence. We found that in dc/2/3/4 triple mutant plants, treatment with exogenous SA stimulated resistance to two positive-sense RNA viruses: cucumber mosaic virus and tobacco mosaic virus. We conclude that DCLs 2, 3 and 4, which are the predominant DCL encloribonucleases involved in silencing of positive-sense RNA viruses, are not required for effective SA-induced resistance to these viruses. However, the findings do not exclude RNA silencing from making a contribution to SA-mediated resistance in wild-type plants.

引用

页码：3010 / 3014

页数：5

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