Biocompatibility of the Biopolymer Cyanoflan for Applications in Skin Wound Healing

被引:13
作者
Costa, Raquel [1 ,2 ]
Costa, Luis [1 ,3 ]
Rodrigues, Ilda [2 ]
Meireles, Catarina [1 ]
Soares, Raquel [1 ,2 ]
Tamagnini, Paula [1 ,3 ,4 ]
Mota, Rita [1 ,3 ]
机构
[1] Univ Porto, Inst Invest & Inovacao Saude I3S, Rua Alfredo Allen 208, P-4200135 Porto, Portugal
[2] Univ Porto, Fac Med, Dept Biomed, Unit Biochem, Al Prof Hernani Monteiro, P-4200319 Porto, Portugal
[3] Univ Porto, IBMC Inst Biol Mol & Celular, Rua Alfredo Allen 208, P-4200135 Porto, Portugal
[4] Univ Porto, Fac Ciencias, Dept Biol, Rua Campo Alegre,Edificio FC4, P-4169007 Porto, Portugal
关键词
antioxidant; cyanobacteria; Cyanoflan; wound healing; wound dressings; skin regeneration;
D O I
10.3390/md19030147
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
There is a great demand for the development of novel wound dressings to overcome the time and costs of wound care performed by a vast number of clinicians, especially in the current overburdened healthcare systems. In this study, Cyanoflan, a biopolymer secreted by a marine unicellular cyanobacterium, was evaluated as a potential biomaterial for wound healing. Cyanoflan effects on cell viability, apoptosis, and migration were assessed in vitro, while the effect on tissue regeneration and biosafety was evaluated in healthy Wistar rats. The cell viability and apoptosis of fibroblasts and endothelial cells was not influenced by the treatment with different concentrations of Cyanoflan, as observed by flow cytometry. Moreover, the presence of Cyanoflan did not affect cell motility and migratory capacity, nor did it induce reactive oxygen species production, even revealing an antioxidant behavior regarding the endothelial cells. Furthermore, the skin wound healing in vivo assay demonstrated that Cyanoflan perfectly adapted to the wound bed without inducing systemic or local oxidative or inflammatory reaction. Altogether, these results suggest that Cyanoflan is a promising biopolymer for the development of innovative applications to overcome the many challenges that still exist in skin wound healing.
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页数:12
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