circRNA-miRNA-mRNA network in age-related macular degeneration: From construction to identification

被引：20

作者：

Su, Yu ^{[1
]}

Yi, Yuexiong ^{[2
]}

Li, Lu ^{[1
]}

Chen, Changzheng ^{[1
]}

机构：

[1] Wuhan Univ, Renmin Hosp, Ctr Eye, Wuhan 430060, Hubei, Peoples R China

[2] Wuhan Univ, Zhongnan Hosp, Dept Obstet & Gynecol, Wuhan 430071, Hubei, Peoples R China

来源：

EXPERIMENTAL EYE RESEARCH | 2021年 / 203卷

基金：

中国国家自然科学基金;

关键词：

Noncoding RNA; Age-related macular degeneration; Bioinformatics; Neurodegeneration disease; PROTEIN-INTERACTION NETWORKS; RETINAL-PIGMENT EPITHELIUM; OXIDATIVE STRESS; CIRCULAR RNAS; GENE-EXPRESSION; R PACKAGE; MICRORNAS; CERNA; NEOVASCULARIZATION; TARGET;

D O I：

10.1016/j.exer.2020.108427

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The aim of the present study was to investigate the pathogenesis of age-related macular degeneration (AMD) by constructing a regulatory circRNA-miRNA-mRNA network. By adjusting the P value to 0.05 and the absolute log value of fold change to 0.25, 2920 and 1057 differentially expressed mRNAs were identified from GSE50195 and GSE29801, respectively. Based on a literature review, Starbase database analysis, and RNA hybrid assays, we obtained 77 miRNA-mRNA and 331 circRNA-miRNA pairs. After combining these pairs, we constructed a circRNA-miRNA-mRNA network possessing 303 circRNA nodes, 4 miRNA nodes, 51 mRNA nodes, and 408 edges. By utilizing protein-protein network analysis, the MCODE algorithm, and the highest degree of circRNA node, we identified the regulatory axis of hsa_circRNA7329/hsa-miR-9/SCD. Hsa_circRNA7329 may regulate SCD through hsa-miR-9 to promote macrophage-mediated inflammation and pathologic angiogenesis, which lead to AMD development. However, the underlying details require further investigation.

引用

页数：12

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