Antigen production and standardization of an in-house indirect ELISA for detection of antibodies against Anaplasma marginale

被引：1
作者：
Salinas Estrella, Elizabeth [1 ]
Ortega Hernandez, Maria Guadalupe [2 ]
Flores Perez, Erika [2 ]
Montenegro Cristino, Natividad [2 ]
Preciado de la Torre, Jesus Francisco [1 ]
Cobaxin Cardenas, Mayra Elizeth [1 ]
Rodriguez, Sergio D. [1 ]
机构：
[1] Ctr Nacl Invest Disciplinaria Salud Anim & Inocui, Inst Nacl Invest Forest Agricolas & Pecuarias INI, Carretera Cuernavaca Cuautla 8534,Col Progreso, Jiutepec 62574, Morelos, Mexico
[2] Serv Nacl Sanidad Inocuidad & Calidad Agroaliment, Jiutepec, Morelos, Mexico
来源：
REVISTA MEXICANA DE CIENCIAS PECUARIAS | 2022年 / 13卷 / 04期
关键词：
Bovine anaplasmosis; Indirect ELISA; Serologic diagnostics; LINKED-IMMUNOSORBENT-ASSAY; INDIRECT FLUORESCENT-ANTIBODY; BABESIA-BIGEMINA; DIAGNOSIS; PROTEIN; TESTS;
D O I：
10.22319/rmcp.v13i4.5976
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Serologic tests are important for the detection of specific antibodies against infectious agents. Commercial indirect ELISA are costly and usually as effective as in-house ELISAs. In the present work, it was prepared a batch of Anaplasma marginale crude antigen from infected blood, and tested it against official positive and negative serum controls and compared with an old batch of antigen. The new antigen batch showed an efficiency similar to the old batch. The sensitivity of the test was comparable between the new and old batches. Both, new and old antigen lots are being used at an excess. The new antigen lot is large enough to run thousands of tests at a more affordable price than commercial kits.
引用
收藏
页码：1079 / 1094
页数：16
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