Plasma protein binding of tetrodotoxin in the marine puffer fish Takifugu rubripes

被引:18
作者
Matsumoto, Takuya [1 ]
Tanuma, Daisuke [1 ]
Tsutsumi, Kazuma [1 ]
Jeon, Joong-Kyun [2 ]
Ishizaki, Shoichiro [1 ]
Nagashima, Yuji [1 ]
机构
[1] Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
[2] Kangnung Wonju Natl Univ, Div Marine Biosci & Technol, Jibyeon 210702, Gangneung, South Korea
基金
日本学术振兴会;
关键词
Tetrodotoxin; Plasma protein binding; Equilibrium dialysis; Marine puffer fish Takifugu rubripes; Greenling Hexagrammos otakii; Bovine serum albumin; Bovine alpha-1-acid glycoprotein; Pharmacokinetics; ACCUMULATION; PURIFICATION; KUSAFUGU; TOXIFICATION; NIPHOBLES; SAXITOXIN; ALBUMIN;
D O I
10.1016/j.toxicon.2009.09.006
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
To elucidate the involvement of plasma protein binding in the disposition of tetrodotoxin (TTX) in puffer fish, we used equilibrium dialysis to measure protein binding of TTX in the plasma of the marine puffer fish Takifugu rubripes and the non-toxic greenling Hexagrammos otakii, and in solutions of bovine serum albumin (BSA) and bovine alpha-1-acid glycoprotein (AGP). TTX (100-1000 mu g/mL) bound to protein in T rubripes plasma with low affinity in a non-saturable manner. The amount of bound TTX increased linearly with the TTX concentration, reaching 3.92 +/- 0.42 mu g TTX/mg protein at 1000 mu g TTX/mL. Approximately 80% of the TTX in the plasma of T rubripes was unbound in the concentration range of TTX examined, indicating that TTX exists predominantly in the unbound form in the circulating blood of T rubripes at a wide range of TrX concentrations. TTX also bound non-specifically to H. otakii plasma proteins, BSA, and bovine AGP. The amount of the bound TTX in the plasma of H. otakii and BSA, respectively, was 1.86 +/- 0.36 and 4.65 +/- 0.70 mu g TTX/mg protein at 1000 mu gTTX/ml, and that in the bovine AGP was 8.78 +/- 0.25 mu gTTX/mg protein at 200 mu gTTX/mL. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:415 / 420
页数:6
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