Genetic diversity among natural populations of Ottelia acuminata (Gaghep.) Dandy revealed by ISSR

被引：0

作者：

Zhang, H. Y. ^{[1
,2
]}

Tian, K. ^{[1
]}

Yu, Y. ^{[2
]}

Li, L. Y. ^{[1
]}

Yang, Y. M. ^{[1
]}

机构：

[1] SW Forestry Univ, Natl Plateau Wetland Res Ctr, Kunming 650224, Yunnan Province, Peoples R China

[2] SW Forestry Univ, Biotechnol Lab, Kunming 650224, Yunnan Province, Peoples R China

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 22期

关键词：

Ottelia acuminate; ISSR; genetic diversity; DNA polymorphism;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ottelia acuminata (Gagnep.) Dandy, an aquatic species of the Hydrocharitaceae, is endemic to China. A performance comparison of genetic diversity of 4 natural populations was conducted to investigate whether or not water pollution in their habitats has anything to do with this species being endangered. A total number of 120 O. acuminate accessions were analyzed, by amplification of their DNAs with 15 primers (ISSR). Thirteen primers were scored and 214 bands were detected, of which 170 were polymorphic (79.44%). The results showed that the genetic indices in polluted Jian Lake group were always the smallest ones, when compared with those of the other groups. It indicated that the polluted water did affect the genetic diversity of O. acuminate populations. And ISSRs seemed to be effective tools for detecting genetic variation among O. acuminate geographical groups.

引用

页码：6089 / 6093

页数：5

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