Involved and Uninvolved Psoriatic Keratinocytes Display a Resistance to Apoptosis that may Contribute to Epidermal Thickness

被引:23
作者
Bivik Eding, Cecilia [1 ]
Enerback, Charlotta [1 ]
机构
[1] Linkoping Univ, Fac Med & Hlth Sci, Dept Clin & Expt Med, Ingrid Asp Psoriasis Res Ctr, SE-58185 Linkoping, Sweden
基金
瑞典研究理事会;
关键词
psoriasis; keratinocytes; apoptosis; GENE-EXPRESSION; INNATE IMMUNITY; SKIN; PROTEIN; BCL-2; P53; PROLIFERATION; FIBRONECTIN; SUNBURN; CELLS;
D O I
10.2340/00015555-2656
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Psoriasis is a common autoimmune skin disease. The aim of this study was to investigate whether the apoptotic process is disturbed in psoriatic keratinocytes. In vitro culture of keratinocytes derived from both involved and uninvolved psoriatic skin, revealed higher viability and resistance to apoptosis following exposure to ultraviolet B, compared with cells from healthy controls. The position of apoptotic dysregulation was found to be upstream of cytochrome c release in the mitochondrial apoptotic pathway. Microarray transcriptome analysis revealed that 87 genes were differentially expressed in both involved and uninvolved psoriatic keratinocytes compared with controls. Among these, a general upregulation of anti-apoptotic genes and downregulation of pro-apoptotic genes were identified. This distinct apoptosis-resistant phenotype, unrelated to the inflammatory component of the disease, implies that intrinsic abnormalities in keratinocytes may contribute to the pathogenesis of psoriasis.
引用
收藏
页码:788 / 796
页数:9
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