Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues

被引:16
作者
Bryant, Aled H. [1 ]
Spencer-Harty, Samantha [2 ]
Owens, Sian-Eleri [1 ]
Jones, Ruth H. [1 ]
Thornton, Catherine A. [1 ]
机构
[1] Swansea Univ, Sch Med, Inst Life Sci, Singleton Pk Campus,Singleton Pk, Swansea SA2 8PP, W Glam, Wales
[2] Abertawe Bro Morgannwg Univ, Hlth Board, Dept Histopathol, Swansea, W Glam, Wales
关键词
inflammation; pregnancy; placenta; IL4; IL13; IL-4; SIGNAL-TRANSDUCTION; INDUCED PRETERM BIRTH; SMOOTH-MUSCLE-CELLS; TOLL-LIKE RECEPTORS; GENE-EXPRESSION; ANTIINFLAMMATORY CYTOKINES; ALVEOLAR MACROPHAGES; NEGATIVE REGULATION; HUMAN FIBROBLASTS; FETAL MEMBRANES;
D O I
10.1095/biolreprod.116.145680
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Inflammation is a key feature of preterm and term labor. Proinflammatory mediators are produced by gestation-associated tissues in response to pathogen-associated molecular patterns and damage-associated molecular patterns. Interleukin (IL)4, IL10, and IL13 are anti-inflammatory cytokines with potential as anti-inflammatory therapies to prevent preterm birth. The objective of this study was to determine if IL4 and IL13 exert anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated production of proinflammatory cytokines produced by human term gestation-associated tissues (placenta, choriodecidua, and amnion). Both IL4 and IL13 reduced LPS-stimulated IL1B and macrophage inflammatory protein1A; this effect diminished with delay to exposure to either cytokine. There was no effect on LPS-stimulated prostaglandin production. Interleukin 4 receptor alpha (IL4RA) was expressed throughout the placenta, choriodecidua, and amnion, and the inhibitory effects of IL4 and IL13 were IL4RA dependent. Combined IL4 and IL13 did not enhance the anti-inflammatory potential of either cytokine; however, a combination of IL4 and IL10 had a greater anti-inflammatory effect than either cytokine alone. These findings demonstrate that human term gestation-associated tissues are responsive to the anti-inflammatory cytokines IL4 and IL13, which could downregulate LPS-induced cytokine production in these tissues. Antiinflammatory cytokines might offer an adjunct to existing therapeutics to prevent adverse obstetric outcome.
引用
收藏
页码:576 / 586
页数:11
相关论文
共 47 条
[1]   IL-13 receptor α 2:: A regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts [J].
Andrews, Allison-Lynn ;
Nasir, Tosia ;
Bucchieri, Fabio ;
Holloway, John W. ;
Holgate, Stephen T. ;
Davies, Donna E. .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2006, 118 (04) :858-865
[2]   IL-4 receptor α is an important modulator of IL-4 and IL-13 receptor binding:: Implications for the development of therapeutic targets [J].
Andrews, Allison-Lynn ;
Holloway, John W. ;
Holgate, Stephen T. ;
Davies, Donna E. .
JOURNAL OF IMMUNOLOGY, 2006, 176 (12) :7456-7461
[3]   Cytoplasmic tail of IL-13Rα2 regulates IL-4 signal transduction [J].
Andrews, Allison-Lynn ;
Nordgren, Ida Karin ;
Kirby, Isabelle ;
Holloway, John W. ;
Holgate, Stephen T. ;
Davies, Donna E. ;
Tavassoli, Ali .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2009, 37 :873-876
[4]  
[Anonymous], SEMIN ONCOL S9
[5]   IL-10 Modulates Placental Responses to TLR Ligands [J].
Bayraktar, Mehmet ;
Peltier, Morgan ;
Vetrano, Anna ;
Arita, Yuko ;
Gurzenda, Ellen ;
Joseph, Ansamma ;
Kazzaz, Jeffrey ;
Sharma, Surendra ;
Hanna, Nazeeh .
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2009, 62 (06) :390-399
[6]   Interleukin 13 inhibits macrophage inflammatory protein-1 alpha production from human alveolar macrophages and monocytes [J].
Berkman, N ;
John, M ;
Roesems, G ;
Jose, P ;
Barnes, PJ ;
Chung, KF .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1996, 15 (03) :382-389
[7]   The regulation of prostaglandin output from term intact fetal membranes by anti-inflammatory cytokines [J].
Brown, NL ;
Alvi, SA ;
Elder, MG ;
Bennett, PR ;
Sullivan, MHF .
IMMUNOLOGY, 2000, 99 (01) :124-133
[8]  
Bryant AH, 2012, CYTOKINES INNATE IMM
[9]   IL-4 inhibition of IL-1 induced Matrix Metalloproteinase-3 (MMP-3) expression in human fibroblasts involves decreased AP-1 activation via negative crosstalk involving of Jun CN-terminal Kinase (JNK) [J].
Chambers, Mariah ;
Kirkpatrick, Garrett ;
Evans, Michel ;
Gorski, Grzegorz ;
Foster, Sara ;
Borghaei, CRuth C. .
EXPERIMENTAL CELL RESEARCH, 2013, 319 (10) :1398-1408
[10]   Regulation of the ant-inflammatory cytokines interleukin-4 and interleukin-10 during pregnancy [J].
Chatterjee, Piyali ;
Chiasson, Valorie L. ;
Bounds, Kelsey R. ;
Mitchell, Brett M. .
FRONTIERS IN IMMUNOLOGY, 2014, 5 :1-1