Inflammation is a key feature of preterm and term labor. Proinflammatory mediators are produced by gestation-associated tissues in response to pathogen-associated molecular patterns and damage-associated molecular patterns. Interleukin (IL)4, IL10, and IL13 are anti-inflammatory cytokines with potential as anti-inflammatory therapies to prevent preterm birth. The objective of this study was to determine if IL4 and IL13 exert anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated production of proinflammatory cytokines produced by human term gestation-associated tissues (placenta, choriodecidua, and amnion). Both IL4 and IL13 reduced LPS-stimulated IL1B and macrophage inflammatory protein1A; this effect diminished with delay to exposure to either cytokine. There was no effect on LPS-stimulated prostaglandin production. Interleukin 4 receptor alpha (IL4RA) was expressed throughout the placenta, choriodecidua, and amnion, and the inhibitory effects of IL4 and IL13 were IL4RA dependent. Combined IL4 and IL13 did not enhance the anti-inflammatory potential of either cytokine; however, a combination of IL4 and IL10 had a greater anti-inflammatory effect than either cytokine alone. These findings demonstrate that human term gestation-associated tissues are responsive to the anti-inflammatory cytokines IL4 and IL13, which could downregulate LPS-induced cytokine production in these tissues. Antiinflammatory cytokines might offer an adjunct to existing therapeutics to prevent adverse obstetric outcome.
机构:
UMDNJ Robert Wood Johnson Med Sch, Dept Pediat Neonatol, New Brunswick, NJ USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Bayraktar, Mehmet
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Peltier, Morgan
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机构:Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Peltier, Morgan
;
Vetrano, Anna
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机构:
UMDNJ Robert Wood Johnson Med Sch, Dept Pediat Neonatol, New Brunswick, NJ USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Vetrano, Anna
;
Arita, Yuko
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机构:Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Arita, Yuko
;
Gurzenda, Ellen
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机构:Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Gurzenda, Ellen
;
Joseph, Ansamma
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机构:
Lenetix Inc, Mineola, NY USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Joseph, Ansamma
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Kazzaz, Jeffrey
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机构:
N Shore LIJ Hlth Syst, Mol Diagnost Lab, Lake Success, NY USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Kazzaz, Jeffrey
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Sharma, Surendra
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机构:
Women & Infants Hosp Rhode Isl, Dept Pediat, Providence, RI 02908 USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Sharma, Surendra
;
Hanna, Nazeeh
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Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
机构:
UMDNJ Robert Wood Johnson Med Sch, Dept Pediat Neonatol, New Brunswick, NJ USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Bayraktar, Mehmet
;
Peltier, Morgan
论文数: 0引用数: 0
h-index: 0
机构:Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Peltier, Morgan
;
Vetrano, Anna
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h-index: 0
机构:
UMDNJ Robert Wood Johnson Med Sch, Dept Pediat Neonatol, New Brunswick, NJ USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Vetrano, Anna
;
Arita, Yuko
论文数: 0引用数: 0
h-index: 0
机构:Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Arita, Yuko
;
Gurzenda, Ellen
论文数: 0引用数: 0
h-index: 0
机构:Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Gurzenda, Ellen
;
Joseph, Ansamma
论文数: 0引用数: 0
h-index: 0
机构:
Lenetix Inc, Mineola, NY USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Joseph, Ansamma
;
Kazzaz, Jeffrey
论文数: 0引用数: 0
h-index: 0
机构:
N Shore LIJ Hlth Syst, Mol Diagnost Lab, Lake Success, NY USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Kazzaz, Jeffrey
;
Sharma, Surendra
论文数: 0引用数: 0
h-index: 0
机构:
Women & Infants Hosp Rhode Isl, Dept Pediat, Providence, RI 02908 USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA
Sharma, Surendra
;
Hanna, Nazeeh
论文数: 0引用数: 0
h-index: 0
机构:
Winthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USAWinthrop Univ Hosp, Div Neonatol, Dept Pediat, Women & Children Res Inst, Mineola, NY 11501 USA