Mangiferin, a naturally occurring polyphenol, mitigates oxidative stress induced premature senescence in human dermal fibroblast cells

被引:11
作者
Kanoi, Rashi [1 ]
Loachan, Pooja [2 ]
Das, Shubhankar [3 ]
Rao, Bola Sadashiva Satish [3 ,4 ]
机构
[1] Manipal Acad Higher Educ, Manipal Sch Life Sci, Dept Biotechnol, Manipal 576104, Karnataka, India
[2] Jammu Univ, Inst Human Genet, Jammu 180006, Jammu & Kashmir, India
[3] Manipal Acad Higher Educ, Manipal Sch Life Sci, Dept Radiat Biol & Toxicol, Manipal 576104, Karnataka, India
[4] Manipal Acad Higher Educ, Directorate Res Off, Manipal 576104, Karnataka, India
关键词
G(0); G(1) arrest; Oxidative stress; Polyphenols; Senescence; HYDROGEN-PEROXIDE; CELLULAR SENESCENCE; MITOCHONDRIAL; MANGO;
D O I
10.1007/s11033-020-06074-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chronic oxidative stress has been associated with several human ailments including the condition of aging. Extensive studies have shown the causal relationship between oxidative stress, aging, and cellular senescence. In this regard, forestalling or preventing senescence could delay the aging process as well as act as an intervention against premature aging. Hence, in the present study, we investigated the anti-senescence potential of Mangiferin (MGN) against Hydrogen peroxide (H2O2) induced premature senescence using human dermal fibroblast cells. Early passage human dermal fibroblasts cells were exposed to H2O2 (10 mu M) for 15 days. In order to assess the anti-senescence property of MGN, cells were preconditioned with MGN (10 mu M / 50 mu M; 2 h) followed by addition of H2O2 (10 mu M)(.) H2O2 mediated induction of premature senescence was accompanied by elevated ROS, lowering of mitochondrial mass and membrane potential, changes in ATP content along with G(0)/G(1) arrest and SA-beta-gal expression. While, conditioning the cells with MGN lowered oxidative burden, stabilized mitochondrial membrane potential / mass and protected the cells against cell cycle arrest, ultimately rendering protection against premature senescence. The present findings showed that MGN might act as a potential cytoprotective nutraceutical that can prolong the onset of chronic oxidative stress mediated premature senescence.
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页码:457 / 466
页数:10
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