Cloning of a new Bacillus thuringiensis cry1I-type crystal protein

被引：19
作者：
Choi, SK
Shin, BS
Kong, EM
Rho, HM
Park, SH
机构：
[1] KRIBB, Genome Res Ctr, Taejon 305600, South Korea
[2] KRIBB, Lab Microbial & Bioproc Engn, Taejon 305600, South Korea
[3] Seoul Natl Univ, Dept Mol Biol, Seoul 151742, South Korea
[4] Seoul Natl Univ, Res Ctr Cell Differentiat, Seoul 151742, South Korea
来源：
CURRENT MICROBIOLOGY | 2000年 / 41卷 / 01期
关键词：
D O I：
10.1007/s002840010093
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new cvy1I-type gene, cry1Id1, was cloned from a B. thuringiensis isolate, and its nucleotide sequence was determined. The deduced amino acid sequence of Cry1Id1 is 89.7%, 87.2%, and 83.4% identical to the Cry1Ia, Cry1Ib, and Cry1Ic proteins, respectively. The upstream sequence of the cry1Id1 structural gene was not functional as promoter in B. subtilis. The Cry1Id1 protein, purified from recombinant E. coli cells, had a toxicity comparable to that of Cry1Ia against Plutella xylostella, but it was significantly less active than Cry1Ia against Bombyx mori. Cry1Id1 was not active against the coleopteran insect, Agelastica coerulea.
引用
收藏
页码：65 / 69
页数：5
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