Gold nanoparticles modified porous silicon chip for SALDI-MS determination of glutathione in cells

被引：23
作者：
Wang, Jing [1 ,2 ]
Jie, Mingsha [1 ]
Li, Haifang [1 ]
Lin, Luyao [1 ]
He, Ziyi [1 ]
Wang, Shiqi [1 ]
Lin, Jin-Ming [1 ]
机构：
[1] Tsinghua Univ, Beijing Key Lab Microanalyt Methods & Instrumenta, Dept Chem, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Grad Sch Shenzhen, Shenzhen 518055, Peoples R China
来源：
TALANTA | 2017年 / 168卷
基金：
中国国家自然科学基金;
关键词：
Porous silicon; Gold nanoparticles; Laser desorption/ionization mass spectrometry; Glutathione; Cells; ASSISTED LASER DESORPTION/IONIZATION; MASS-SPECTROMETRY; TRANSPORTER; IRINOTECAN; CARBON;
D O I：
10.1016/j.talanta.2017.02.041
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The gold nanoparticles (Au NPs) modified porous silicon chip based surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) was developed to capture and analyze glutathione (GSH) in cells. With silver assisted chemical etching, Ag nanoparticles (Ag NPs) were generated and deposited on the silicon surface and the nanopores were etched on silicon substrate. Then Au NPs were in-situ synthesized on the ridges of silicon nanopores. This Ag-Au NPs modified porous silicon surface could specially capture and enrich thiol compounds through Au-S binding, and it could also function as matrix to assist ionization for SALDI-MS. The sfficon chip was array patterned for high throughput SALDI-MS detection. GSH and cysteine could be distinguished without the interference from matrix signals. This approach was successfully applied to preconcentration and detection of GSH in Caco-2 cells. The GSH alterations in cells under drug stimulation were investigated. This invented silicon chip showed great potential for more efficient analysis of small thiol biomarkers in complex biological samples.
引用
收藏
页码：222 / 229
页数：8
相关论文
共 35 条
[1]   Reduced glutathione in hepatitis E infection and pregnancy outcome [J].
Bhatnagar, Gunjan ;
Sharma, Sheetal ;
Kumar, Ashok ;
Prasad, Sudha ;
Agarwal, Sarita ;
Kar, Premashish .
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 2016, 42 (07) :789-795
[2]   Gold nanoparticles as a substrate in bio-analytical near-infrared surface-enhanced Raman spectroscopy [J].
Butler, Holly J. ;
Fogarty, Simon W. ;
Kerns, Jemma G. ;
Martin-Hirsch, Pierre L. ;
Fullwood, Nigel J. ;
Martin, Francis L. .
ANALYST, 2015, 140 (09) :3090-3097
[3]  
Camps C., 2010, NANOMED-NANOTECHNOL, V6, P530
[4]   Ratios of biliary glutathione disulfide (GSSG) to glutathione (GSH): a potential index to screen drug-induced hepatic oxidative stress in rats and mice [J].
Cao, Lei ;
Waldon, Daniel ;
Teffera, Yohannes ;
Roberts, John ;
Wells, Mary ;
Langley, Meghan ;
Zhao, Zhiyang .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (08) :2635-2642
[5]   MALDI-MS detection of noncovalent interactions of single stranded DNA with Escherichia coli single-stranded DNA-binding protein [J].
Chen, Fan ;
Maedler, Stefanie ;
Weidmann, Simon ;
Zenobi, Renato .
JOURNAL OF MASS SPECTROMETRY, 2012, 47 (05) :560-566
[6]   Detection of aminothiols through surface-assisted laser desorption/ionization mass spectrometry using mixed gold nanoparticles [J].
Chiang, Ni-Chen ;
Chiang, Cheng-Kang ;
Lin, Zong-Hong ;
Chiu, Tai-Chia ;
Chang, Huan-Tsung .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2009, 23 (19) :3063-3068
[7]   Downregulation of Cystine Transporter xc- by Irinotecan in Human Head and Neck Cancer FaDu Xenografts [J].
Chintala, Sreenivasulu ;
Toth, Karoly ;
Yin, Ming-Biao ;
Bhattacharya, Arup ;
Smith, Sylvia B. ;
Ola, M. Shamsul ;
Cao, Shousong ;
Durrani, Farukh A. ;
Zinia, Tanjima R. ;
Dean, Rebecca ;
Slocum, Harry K. ;
Rustum, Youcef M. .
CHEMOTHERAPY, 2010, 56 (03) :223-233
[8]  
Chu XY, 1997, J PHARMACOL EXP THER, V281, P304
[9]   Ordered silicon nanocavity arrays in surface-assisted desorption/ionization mass spectrometry [J].
Finkel, NH ;
Prevo, BG ;
Velev, OD ;
He, L .
ANALYTICAL CHEMISTRY, 2005, 77 (04) :1088-1095
[10]  
Fotso F.A., 2014, PLOS NEGLECT TROP D, V8, P337
1234