Nucleic Acid Binding and Invitro Cytotoxicity Studies of Polymer Grafted Intercalating Copper(II) Complex

The present study on interaction of a polymer-copper(II) complex, [Cu(dpqC)(2)(BPEI)](ClO4)(2)center dot H2O (dpqC = dipyrido[3,2-a:2',3'-c](6,7,8,9-tetrahydro) phenazine) with CT-DNA/yeast tRNA were performed by using various spectroscopic methods. The data from these experiments suggest that the polymer-copper(II) complex binds to CT-DNA/yeast tRNA through intercalation. Furthermore, our polymer copper(II) complex showed strong binding ability with yeast tRNA than CT-DNA. It indicates that structural differences of the nucleic acids play a significant role in the binding affinity. The antiproliferative and cell death properties of this complex have been tested against MDA-MB-231 breast cancer cell line by various specific staining methods. These obtained results indicate that our complex is more cytotoxic to MDA-MB-231 breast cancer cell line.