Aligned fibrous decellularized cell derived matrices for mesenchymal stem cell amplification

被引:17
作者
Ventre, Maurizio [1 ,2 ,3 ]
Coppola, Valerio [1 ]
Natale, Carlo F. [2 ]
Netti, Paolo A. [1 ,2 ,3 ]
机构
[1] Univ Naples Federico II, Dept Chem Mat & Ind Prod Engn, Ple Tecchio 80, I-80125 Naples, Italy
[2] Univ Naples Federico II, Interdisciplinary Res Ctr Biomat, Naples, Italy
[3] Ist Italiano Tecnol, Ctr Adv Biomat Healthcare CRIB, Naples, Italy
关键词
aligned fibers; cell derived matrices; decellularization; mesenchymal stem cells; EXTRACELLULAR-MATRIX; EX-VIVO; DIFFERENTIATION; GROWTH; TISSUE; EXPANSION; SURFACES;
D O I
10.1002/jbm.a.36759
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Biochemical and biophysical stimuli of stem cell niches finely regulate the self-renewal/differentiation equilibrium. Replicating this in vitro is technically challenging, making the control of stem cell functions difficult. Cell derived matrices capture certain aspect of niches that influence fate decisions. Here, aligned fibrous matrices synthesized by MC3T3 cells were produced and the role of matrix orientation and stiffness on the maintenance of stem cell characteristics and adipo- or osteo-genic differentiation of murine mesenchymal stem cells (mMSCs) was investigated. Decellularized matrices promoted mMSC proliferation. Fibrillar alignment and matrix stiffness work in concert in defining cell fate. Soft matrices preserve stemness, whereas stiff ones, in presence of biochemical supplements, promptly induce differentiation. Matrix alignment impacts the homogeneity of the cell population, that is, soft aligned matrices ameliorate the spontaneous adipogenic differentiation, whereas stiff aligned matrices reduce cross-differentiation. We infer that mechanical signaling is a dominant factor in mMSC fate decision and the matrix alignment contributes to produce a more homogeneous environment, which results in a uniform response of cells to biophysical environment. Matrix thus produced can be obtained in vitro in a facile and consistent manner and can be used for homogeneous stem cell amplification or for mechanotransduction-related studies.
引用
收藏
页码:2536 / 2546
页数:11
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