Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function

被引：56

作者：

Houbrechts, Anne M. ^{[1
]}

Vergauwen, Lucia ^{[2
]}

Bagci, Enise ^{[2
,3
]}

Van Houcke, Jolien ^{[1
]}

Heijlen, Marjolein ^{[1
]}

Kulemeka, Bernard ^{[4
,5
]}

Hyde, David R. ^{[4
,5
]}

Knapen, Dries ^{[2
]}

Darras, Veerle M. ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Lab Comparat Endocrinol, Dept Biol, Div Anim Physiol & Neurobiol, B-3000 Leuven, Belgium

[2] Univ Antwerp, Dept Vet Sci, Zebrafishlab, Vet Physiol & Biochem, Univ Pl 1, B-2610 Antwerp, Belgium

[3] Univ Antwerp, Dept Biol, System Physiol & Ecotoxicol Res, Univ Pl 1, B-2610 Antwerp, Belgium

[4] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

[5] Univ Notre Dame, Ctr Zebrafish Res, Notre Dame, IN 46556 USA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2016年 / 424卷 / 0C期

关键词：

Thyroid hormone; Deiodinase; Morpholino knockdown; Zebrafish; Development; Retina; THYROID-HORMONE RECEPTOR; RETINOIC ACID; CONE PHOTORECEPTORS; OPTOKINETIC RESPONSE; OPSIN EXPRESSION; BINDING PROTEINS; HYPOTHYROIDISM; GROWTH; TRIIODOTHYRONINE; DIFFERENTIATION;

D O I：

10.1016/j.mce.2016.01.018

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Retinal development in vertebrates relies extensively on thyroid hormones. Their local availability is tightly controlled by several regulators, including deiodinases (Ds). Here we used morpholino technology to explore the roles of Ds during eye development in zebrafish. Transcriptome analysis at 3 days post fertilization (dpf) revealed a pronounced effect of knockdown of both T-4-activating Ds (D1D2M0) or knockdown of T-3-inactivating D3 (D3bMO) on phototransduction and retinoid recycling. This was accompanied by morphological defects (studied from 1 to 7 dpf) including reduced eye size, disturbed retinal lamination and strong reduction in rods and all four cone types. Defects were more prominent and persistent in D3-deficient fish. Finally, D3-deficient zebrafish larvae had disrupted visual function at 4 dpf and were less sensitive to a light stimulus at 5 dpf. These data demonstrate the importance of TH-activating and -inactivating Ds for correct zebrafish eye development, and point to D3b as a central player. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

引用

页码：81 / 93

页数：13