Impact of Globule Unfolding on Dilational Viscoelasticity of β-Lactoglobulin Adsorption Layers

被引：48

作者：

Noskov, B. A. ^{[1
]}

Grigoriev, D. O. ^{[2
]}

Latnikova, A. V. ^{[1
]}

Lin, S. -Y. ^{[3
]}

Loglio, G. ^{[4
]}

Miller, R. ^{[2
]}

机构：

[1] St Petersburg State Univ, Fac Chem, St Petersburg 198904, Russia

[2] MPI Kolloid & Grenzflachenforsch, D-14476 Golm, Germany

[3] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 106, Taiwan

[4] Univ Florence, Dipartimento Chim Organ, I-50019 Florence, Italy

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2009年 / 113卷 / 40期

关键词：

AIR-WATER-INTERFACE; DYNAMIC SURFACE-PROPERTIES; AIR/WATER INTERFACE; STRUCTURAL CONFORMATION; LIQUID INTERFACES; NEUTRON REFLECTION; PROTEIN ADSORPTION; POLYMER-SOLUTIONS; LYSOZYME LAYERS; FILM FORMATION;

D O I：

10.1021/jp905413q

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The dynamic surface dilational elasticity, surface Pressure, and adsorbed amount of the mixed solutions Of beta-lactoglobulin and guanidine hydrochloride were measured as a function of surface age and denaturant concentration. It was shown that the conformational transition from compact globules to disordered protein molecules ill the surface layer leads to strong changes in the surface elasticity kinetic dependencies and thereby can be easily detected by measuring the surface dilational rheological properties. The corresponding changes of the kinetic dependencies of the surface pressure and adsorbed amount are not so pronounced but correlate with the results on Surface dilational elasticity.

引用

页码：13398 / 13404

页数：7

共 55 条

[1]

[Anonymous], 2002, PROTEIN PHYS

[2] NEUTRON REFLECTIVITY OF ADSORBED BETA-CASEIN AND BETA-LACTOGLOBULIN AT THE AIR/WATER INTERFACE [J].

ATKINSON, PJ ;

DICKINSON, E ;

HORNE, DS ;

RICHARDSON, RM .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1995, 91 (17) :2847-2854

[3] Compression/expansion rheology of oil/water interfaces with adsorbed proteins. Comparison with the air/water surface [J].

Benjamins, J. ;

Lyklema, J. ;

Lucassen-Reynders, E. H. .

LANGMUIR, 2006, 22 (14) :6181-6188

[4] Protein adsorption at the oil/water interface: characterization of adsorption kinetics by dynamic interfacial tension measurements [J].

Beverung, CJ ;

Radke, CJ ;

Blanch, HW .

BIOPHYSICAL CHEMISTRY, 1999, 81 (01) :59-80

[5] Flow injection analysis with diode array absorbance detection and dynamic surface tension detection for studying denaturation and surface activity of globular proteins [J].

Bramanti, E ;

Allegrini, C ;

Onor, M ;

Raspi, G ;

Skogerboe, KJ ;

Synovec, RE .

ANALYTICAL BIOCHEMISTRY, 2006, 351 (01) :100-113

[6] Kinetics of Adsorption Layer Formation in Solutions of Polyacid/Surfactant Complexes [J].

Bykov, Alexey G. ;

Lin, Shi-Yow ;

Loglio, Giuseppe ;

Miller, Reinhard ;

Noskov, Boris A. .

JOURNAL OF PHYSICAL CHEMISTRY C, 2009, 113 (14) :5664-5671

[7]

Cho D, 1999, B KOREAN CHEM SOC, V20, P999

[8] Protein denaturation in foam - I. Mechanism study [J].

Clarkson, JR ;

Cui, ZF ;

Darton, RC ;

Clarkson, JR .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1999, 215 (02) :323-332

[9] Adsorption dynamics of α-lactalbumin and β-lactoglobulin at air-water interfaces [J].

Cornec, M ;

Cho, D ;

Narsimhan, G .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1999, 214 (02) :129-142

[10] Effect of contaminant on adsorption of whey proteins at the air-water interface [J].

Cornec, M ;

Narsimhan, G .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (07) :2490-2498

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