Facile construction of beads-on-a-string complexes of polymeric micelles and DNA via tunable electrostatic interactions

被引：2

作者：

Zhang, Yifan ^{[1
]}

Li, Xiaojie ^{[1
]}

Wei, Wei ^{[1
]}

Liu, Xiaoya ^{[1
]}

机构：

[1] Jiangnan Univ, Key Lab Synthet & Biol Colloids, Minist Educ, Sch Chem & Mat Engn, Wuxi, Jiangsu, Peoples R China

来源：

COLLOID AND INTERFACE SCIENCE COMMUNICATIONS | 2021年 / 41卷

基金：

中国国家自然科学基金;

关键词：

Chromatin-mimic assembly; Beads-on-a-string complexes; DNA compaction; Poly(tertiary amine methacrylate) micelles; Tunable electrostatic interaction;

D O I：

10.1016/j.colcom.2021.100366

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Inspired by the structure of chromatin fibers, the compaction of DNA by cationic nanoparticles has received much attention in the fields of nanomaterials and biotechnology. However, the fabrication of artificial chromatin-biomimetic assemblies of DNA and nanoparticles in biological environments is still a challenge due to their dependence on uncontrollable electrostatic interactions. Herein, block and random copolymerization of 2-(diisopropylamino)ethyl methacrylate (DPA) and 2-(dimethylamino)ethyl methacrylate (DMA) were used to regulate the charge density and distribution of cationic polyethylene glycol-b-poly(tertiary amine methacrylate) micelles, adjusting the electrostatic interaction between the micelles and DNA. We showed that the assembled PEG-b-PDMA-b-PDPA micelle/DNA system exhibited a strong electrostatic interactions between the micelles and DNA, causing uncontrollable intrachain flocculation. However, the assembled PEG-b-P(DMA-r-DPA) micelle/ DNA system showed relatively week, tunable electrostatic interactions, leading to an open -chromatin-like structure

引用

页数：5