Satellite cell characterization from aging human muscle

被引:44
作者
Corbu, Arianna [1 ,2 ]
Scaramozza, Annarita [1 ,2 ]
Badiali-DeGiorgi, Lucilla [1 ]
Tarantino, Lucia [1 ]
Papa, Valentina [1 ]
Rinaldi, Rita [3 ]
D'Alessandro, Roberto [3 ]
Zavatta, Marcello [4 ]
Laus, Massimo [4 ]
Lattanzi, Giovanna [5 ]
Cenacchi, Giovanna [1 ]
机构
[1] Univ Bologna, Clin Dept Radiol & Histopathol Sci, I-40138 Bologna, Italy
[2] St Orsola Malpighi Univ Hosp, Ctr Appl Biomed Res, Bologna, Italy
[3] St Orsola Malpighi Univ Hosp, Neurol Unit, Bologna, Italy
[4] Azienda Osped Univ, UO Ortopedia Laus, Bologna, Italy
[5] IOR, Cell Biol Lab, Bologna, Italy
关键词
Aging muscle; culture of SCs; human surgery fragment; isolation of satellite cell; satellite cell; SKELETAL-MUSCLE; PROGENITOR CELLS; AGE; PROLIFERATION; INJURY; ADULT; YOUNG; DIFFERENTIATION; REGENERATION; RATS;
D O I
10.1179/174313209X385725
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Objectives: Satellite cells (SCs) are skeletal muscle progenitor cells located between the basal lamina and the sarcolemma of muscle fibers. They are responsible for muscle growth and repair. In humans, aging results in the depletion of the SC population and in its proliferative activity, but not in its function. It has not yet been determined whether under conditions of massive muscle fiber death in vivo, the regenerative potential of SCs is totally or partially compromised in old muscle. No studies have yet tested whether advanced age is a factor that restrains the response of SCs to muscle denervation in humans; this is also due to difficulties in the isolation and in the culture of SCs from a small human surgery fragment. The aim of this study was to study in depth muscle regeneration analysing the SC ability of SCs to proliferate and differentiate in aging human patients. Methods: In order to study in more detail the molecular mechanism, the proliferative and differentiative ability of aging SCs, we isolated SCs from aging human muscle biopsies and analysed their morphology by transmission electron microscopy and immunocytochemical analysis (antibodies against desmin, N-CAM and M-cadherin) and their capacity to grow and to expand in vitro. Moreover, in order to evaluate gene expression of myogenic regulatory factors Myf5, MyoD and myogenin (Myf4), RT-PCR was performed. Results and discussion: SCs isolated from aging human muscle biopsies and plated into favorable proliferation and differentiation conditions were able to proceed through the myogenic program and actively form myotubes, although taking longer than the young control sample. The RT-PCR analysis together with the ultrastructural SC features showed that the myogenic potential seemed to be compromised during the aging human muscle proliferation in vitro.
引用
收藏
页码:63 / 72
页数:10
相关论文
共 37 条
[1]  
ALLBROOK DB, 1971, PATHOLOGY, V3, P233
[2]   Increased myogenic repressor Id mRNA and protein levels in hindlimb muscles of aged rats [J].
Alway, SE ;
Degens, H ;
Lowe, DA ;
Krishnamurthy, G .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2002, 282 (02) :R411-R422
[3]   Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation [J].
Asakura, A ;
Komaki, M ;
Rudnicki, MA .
DIFFERENTIATION, 2001, 68 (4-5) :245-253
[4]   Culture of skeletal myoblasts from human donors aged over 40 years: dynamics of cell growth and expression of differentiation markers [J].
Baj, A ;
Bettaccini, AA ;
Casalone, R ;
Sala, A ;
Cherubino, P ;
Toniolo, AQ .
JOURNAL OF TRANSLATIONAL MEDICINE, 2005, 3 (1)
[5]   A SATELLITE CELL MITOGEN FROM CRUSHED ADULT MUSCLE [J].
BISCHOFF, R .
DEVELOPMENTAL BIOLOGY, 1986, 115 (01) :140-147
[6]  
Bischoff R, 2004, MYOLOGY, V1, P66
[7]   The magnitude of the initial injury induced by stretches of maximally activated muscle fibres of mice and rats increases in old age [J].
Brooks, SV ;
Faulkner, JA .
JOURNAL OF PHYSIOLOGY-LONDON, 1996, 497 (02) :573-580
[8]  
CARLSON BM, 1995, J GERONTOL A-BIOL, V50, P96
[9]  
CARLSON BM, 1989, AM J PHYSIOL, V256, pC1262
[10]   Skeletal muscle regeneration in very old rats [J].
Carlson, BM ;
Dedkov, EI ;
Borisov, AB ;
Faulkner, JA .
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2001, 56 (05) :B224-B233