Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution

被引：13

作者：

Dong, Zhen-Zhen ^{[1
]}

Lu, Lihua ^{[1
,2
]}

Wang, Wanhe ^{[1
]}

Li, Guodong ^{[3
]}

Kang, Tian-Shu ^{[3
]}

Han, Quanbin ^{[4
]}

Leung, Chung-Hang ^{[3
]}

Ma, Dik-Lung ^{[1
,5
]}

机构：

[1] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China

[2] Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China

[3] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China

[4] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China

[5] Hong Kong Baptist Unvers, Inst Res & Continuing Educ, Shenzhen 518057, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 246卷

基金：

中国国家自然科学基金;

关键词：

Iridium(III) complex; Nicking endonuclease; Luminescence; G-quadruplex; Probe; CYCLOMETALATED IR(III) COMPLEXES; LABEL-FREE DETECTION; SWITCH-ON DETECTION; SIGNAL AMPLIFICATION; STRAND DISPLACEMENT; DNA DETECTION; FLUORESCENCE; ASSAY; STRATEGY; LIGANDS;

D O I：

10.1016/j.snb.2017.02.156

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.Bsml as a model enzyme. In the assay, an initial hairpin DNA (5'-TG(4)AG(3)TG(4)AG(3)TG(4)A(2)G(3)A(2)TGCTA(3)TAGCAT(2)C(3)T(2)C(4)AC-3') sequence containing the Nb.Bsml recognition site in its stem region and a partially caged G-rich DNA sequence at its 5'-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.Bsml concentration over the range of 0-10 U/mL (R-2=0.9937) with a detection limit for Nb.Bsml of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.Bsmi over other nicking endonucleases, and functioned effectively in the presence of cell lysates. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：826 / 832

页数：7

共 62 条

[11] Modulating Fluorescence Anisotropy of Terminally Labeled Double-Stranded DNA via the Interaction between Dye and Nucleotides for Rational Design of DNA Recognition Based Applications [J].

Huang, Hongduan ;

Wei, Hejia ;

Zou, Mingjian ;

Xu, Xiao ;

Xia, Bin ;

Liu, Feng ;

Li, Na .

ANALYTICAL CHEMISTRY, 2015, 87 (05) :2748-2754

[12] G-quadruplex DNAzyme-based Hg2+ and cysteine sensors utilizing Hg2+-mediated oligonucleotide switching [J].

Jia, Shu-Min ;

Liu, Xiao-Fei ;

Li, Ping ;

Kong, De-Ming ;

Shen, Han-Xi .

BIOSENSORS & BIOELECTRONICS, 2011, 27 (01) :148-152

[13] Linear nicking endonuclease-mediated strand-displacement DNA amplification [J].

Joneja, Aric ;

Huang, Xiaohua .

ANALYTICAL BIOCHEMISTRY, 2011, 414 (01) :58-69

[14] Identification of an Iridium(III)-Based Inhibitor of Tumor Necrosis Factor-α [J].

Kang, Tian-Shu ;

Mao, Zhifeng ;

Ng, Chan-Tat ;

Wang, Modi ;

Wang, Wanhe ;

Wang, Chunming ;

Lee, Simon Ming-Yuen ;

Wang, Yitao ;

Leung, Chung-Hang ;

Ma, Dik-Lung .

JOURNAL OF MEDICINAL CHEMISTRY, 2016, 59 (08) :4026-4031

[15] Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA) [J].

Kiesling, Traci ;

Cox, Kendra ;

Davidson, Eugene A. ;

Dretchen, Kenneth ;

Grater, Guy ;

Hibbard, Shannon ;

Lasken, Roger S. ;

Leshin, Jonathan ;

Skowronski, Evan ;

Danielsen, Mark .

NUCLEIC ACIDS RESEARCH, 2007, 35 (18)

[16] Discrimination of G-Quadruplexes from Duplex and Single-Stranded DNAs with Fluorescence and Energy-Transfer Fluorescence Spectra of Crystal Violet [J].

Kong, De-Ming ;

Ma, Yong-E. ;

Wu, Jing ;

Shen, Han-Xi .

CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (04) :901-909

[17] Artificial site-specific DNA-nicking system based on common restriction enzymes assisted by PNA openers [J].

Kuhn, H ;

Hu, YB ;

Frank-Kamenetskii, MD ;

Demidov, VV .

BIOCHEMISTRY, 2003, 42 (17) :4985-4992

[18] Circular dichroism and conformational polymorphism of DNA [J].

Kypr, Jaroslav ;

Kejnovska, Iva ;

Renciuk, Daniel ;

Vorlickova, Michaela .

NUCLEIC ACIDS RESEARCH, 2009, 37 (06) :1713-1725

[19] Label-Free Luminescent Switch-on Detection of Endonuclease IV Activity Using a G-Quadruplex-Selective Iridium(III) Complex [J].

Leung, Ka-Ho ;

He, Hong-Zhang ;

Wang, Wei ;

Zhong, Hai-Jing ;

Chan, Daniel Shiu-Hin ;

Leung, Chung-Hang ;

Ma, Dik-Lung .

ACS APPLIED MATERIALS & INTERFACES, 2013, 5 (23) :12249-12253

[20] A dinuclear iridium(III) complex as a visual specific phosphorescent probe for endogenous sulphite and bisulphite in living cells [J].

Li, Guanying ;

Chen, Yu ;

Wang, Jinquan ;

Lin, Qian ;

Zhao, Jing ;

Ji, Liangnian ;

Chao, Hui .

CHEMICAL SCIENCE, 2013, 4 (12) :4426-4433

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