A novel and an effective analytical approach for the LC-MS determination of ethyl glucuronide and ethyl sulfate in urine

被引：22
|
作者：
Favretto, Donata [1 ]
Nalesso, Alessandro [1 ]
Frison, Giampietro [1 ]
Viel, Guido [1 ]
Traldi, Pietro [2 ]
Ferrara, Santo Davide [1 ]
机构：
[1] Univ Hosp Padova, I-35121 Padua, Italy
[2] CNR, Inst Mol Sci & Technol, I-35100 Padua, Italy
来源：
INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2010年 / 124卷 / 02期
关键词：
Liquid chromatography; Mass sprectrometry; Ethyl glucuronide; Ethyl sulfate; PRESSURE CHEMICAL-IONIZATION; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; HUMAN SERUM; ELECTROSPRAY-IONIZATION; ALCOHOL-CONSUMPTION; ETHANOL METABOLITE; ELECTROPHORESIS; HUMANS;
D O I：
10.1007/s00414-009-0376-1
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
An alternative liquid chromatography-mass spectrometry (LC-MS) method based on no discharge (ND) atmospheric pressure chemical ionization (APCI) was developed for the simultaneous determination of ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine in negative ion conditions. Abundant [M-H](-) species of EtG and EtS were obtained, allowing to reach limits of quantification (0.1 A mu g/ml for both analytes), accuracy, and precision comparable to those proposed in the literature. Additionally, the LC-ND-APCI-MS method proved to be reliable, requiring little maintenance even when high throughput analyses (i.e., 6,000 samples per year) were required.
引用
收藏
页码：161 / 164
页数：4
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