Phosphorus removal by the Ceratophyllum/periphyton complex in a south Florida (USA) freshwater marsh

Removal of phosphorus (P) by Ceratophyllum demersum L. and associated epiphytic periphyton was quantified by measuring the disappearance of soluble reactive P (SRP) from microcosms during 1-h in situ incubations conducted over a 1-year period. Initial P concentrations in these incubations ranged from 30 to >10,000 mu g P L-1. Phosphorus removal was proportional to initial P concentrations and was weakly correlated with solar irradiance and water temperature. Removal rates (0.6-32.8 mg p m(-2) d(-1)) and k(v) coefficients (0.68-1.93 h(-1)) from experiments run at low initial P concentrations (up to 200 mu g P L-1) were comparable to results reported for other macrophytes. Removal rates from experiments run at the highest (>10,000 mu g P L-1) initial P concentrations (5300 and 11,100 mg p m(-2) d(-1)) most likely represented luxury nutrient consumption and were not thought to be sustainable long term. We were unable to determine a V-max for P removal, suggesting that the nutrient-storage capability of the C. demersum/periphyton complex was not saturated during our short-term incubations. Based on N:P molar ratios, the marsh was P limited, while the C. demersum/periphyton complex was either N limited or in balance for N and P throughout this study. However, despite its tissue stoichiometry, the C. demersum/periphyton complex always exhibited an affinity for P. It appeared that the biochemical mechanisms, which mediate P removal, at least on a short-term basis, were more influenced by increases in ambient P levels than by tissue nutrient stoichiometry. (C) 2006 Elsevier B.V. All rights reserved.