Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae

被引：7
作者：
Thurman, Kathleen A. [1 ]
Cowart, Kelley C. [1 ]
Winchell, Jonas M. [1 ]
机构：
[1] Ctr Dis Control & Prevent, Resp Dis Branch, Atlanta, GA 30329 USA
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 65卷 / 04期
关键词：
Mycoplasma pneumoniae; Nucleic acid extraction; Community-acquired pneumonia; POLYMERASE-CHAIN-REACTION; INFECTION; DIAGNOSIS; OUTBREAK;
D O I：
10.1016/j.diagmicrobio.2009.08.001
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Four nucleic acid extraction procedures (2 automated and 2 manual) were compared for their efficiency at isolating Mycoplasma pneumoniae DNA. Oropharyngeal swabs from healthy volunteers were spiked with varying amounts of M. pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently outperform the manual procedures. Published by Elsevier Inc.
引用
收藏
页码：435 / 438
页数：4
相关论文
共 12 条
  • [1] Laboratory diagnosis of Mycoplasma pneumoniae infection
    Daxboeck, F
    Krause, R
    Wenisch, C
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2003, 9 (04) : 263 - 273
  • [2] Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus
    Emery, SL
    Erdman, DD
    Bowen, MD
    Newton, BR
    Winchell, JM
    Meyer, RF
    Tong, SX
    Cook, BT
    Holloway, BP
    McCaustland, KA
    Rota, PA
    Bankamp, B
    Lowe, LE
    Ksiazek, TG
    Bellini, WJ
    Anderson, LJ
    [J]. EMERGING INFECTIOUS DISEASES, 2004, 10 (02) : 311 - 316
  • [3] IDENTIFICATION OF A POSSIBLE CYTADHERENCE REGULATORY LOCUS IN MYCOPLASMA-PNEUMONIAE
    HEDREYDA, CT
    KRAUSE, DC
    [J]. INFECTION AND IMMUNITY, 1995, 63 (09) : 3479 - 3483
  • [4] Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs
    Loens, K.
    Ursi, D.
    Goossens, H.
    Leven, M.
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 72 (02) : 217 - 219
  • [5] Evaluation of NucliSens easyMAG for automated nucleic acid extraction from various clinical specimens
    Loens, K.
    Bergs, K.
    Ursi, D.
    Goossens, H.
    Ieven, M.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) : 421 - 425
  • [6] Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections
    Loens, K
    Ursi, D
    Goossens, H
    Ieven, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) : 4915 - 4923
  • [7] MADELEY CR, 1988, LAB DIAGNOSIS INFECT, V2, P1
  • [8] Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection
    Nilsson, Anna C.
    Bjorkman, Per
    Persson, Kenneth
    [J]. BMC MICROBIOLOGY, 2008, 8 (1)
  • [9] Comparison of Laboratory Diagnostic Procedures for Detection of Mycoplasma pneumoniae in Community Outbreaks
    Thurman, Kathleen A.
    Walter, Nicholas D.
    Schwartz, Stephanie B.
    Mitchell, Stephanie L.
    Dillon, Michael T.
    Baughman, Andrew L.
    Deutscher, Meredith
    Fulton, John P.
    Tongren, Jon E.
    Hicks, Lauri A.
    Winchell, Jonas M.
    [J]. CLINICAL INFECTIOUS DISEASES, 2009, 48 (09) : 1244 - 1249
  • [10] Mycoplasma pneumoniae and its role as a human pathogen
    Waites, KB
    Talkington, DF
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (04) : 697 - +
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