Study of biochemical pathways and enzymes involved in pyrene degradation by Mycobacterium sp strain KMS

被引:84
作者
Liang, Yanna
Gardner, Dale R.
Miller, Charles D.
Chen, Dong
Anderson, Anne J.
Weimer, Bart C.
Sims, Ronald C.
机构
[1] Utah State Univ, Dept Biol & Irrigat Engn, Logan, UT 84322 USA
[2] Utah State Univ, Dept Civil & Environm Engn, Logan, UT 84322 USA
[3] USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA
[4] Utah State Univ, Dept Biol, Logan, UT 84322 USA
[5] Utah State Univ, Ctr Integrated Biosyst, Logan, UT 84322 USA
关键词
POLYCYCLIC AROMATIC-HYDROCARBON; POLYACRYLAMIDE-GEL ELECTROPHORESIS; DEGRADING MYCOBACTERIUM; VANBAALENII PYR-1; IDENTIFICATION; DIOXYGENASES; PROTEINS; CLONING; SOIL; PHENANTHRENE;
D O I
10.1128/AEM.01274-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pyrene degradation is known in bacteria. In this study, Mycobacterium sp. strain KMS was used to study the metabolites produced during, and enzymes involved in, pyrene degradation. Several key metabolites, including pyrene-4,5-dione, cis-4,5-pyrene-dihydrodiol, phenanthrene-4,5-dicarboxylic acid, and 4-phenanthroic acid, were identified during pyrene degradation. Pyrene-4,5-dione, which accumulates as an end product in some gram-negative bacterial cultures, was further utilized and degraded by Mycobacterium sp. strain KMS. Enzymes involved in pyrene degradation by Mycobacterium sp. strain KMS were studied, using 2-D gel electrophoresis. The first protein in the catabolic pathway, aromatic-ring-hydroxylating dioxygenase, which oxidizes pyrene to cis-4,5-pyrene-dihydrodiol, was induced with the addition of pyrene and pyrene-4,5-dione to the cultures. The subcomponents of dioxygenase, including the alpha and beta subunits, 4Fe-4S ferredoxin, and the Rieske (2Fe-2S) region, were all induced. Other proteins responsible for further pyrene degradation, such as dihydrodiol dehydrogenase, oxidoreductase, and epoxide hydrolase, were also found to be significantly induced by the presence of pyrene and pyrene-4,5-dione. Several nonpathway-related proteins, including sterol-binding protein and cytochrome P450, were induced. A pyrene degradation pathway for Mycobacterium sp. strain KMS was proposed and confirmed by proteomic study by identifying almost all the enzymes required during the initial steps of pyrene degradation.
引用
收藏
页码:7821 / 7828
页数:8
相关论文
共 33 条
[1]   Molecular characterization of dioxygenases from polycyclic aromatic hydrocarbon-degrading Mycobacterium spp. [J].
Brezna, B ;
Khan, AA ;
Cerniglia, CE .
FEMS MICROBIOLOGY LETTERS, 2003, 223 (02) :177-183
[2]  
Brezna B., 2005, APPL MICROBIOL BIOT, V11, P1
[3]  
Cerniglia Carl E., 1992, Biodegradation, V3, P351, DOI 10.1007/BF00129093
[4]   Degradation of pyrene by Mycobacterium flavescens [J].
DeanRoss, D ;
Cerniglia, CE .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 46 (03) :307-312
[5]  
FARA F, 2004, BIOTECHNOL BIOENG, V88, P214
[6]   The effect of aging on pyrene transformation in sediments [J].
Guthrie-Nichols, E ;
Grasham, A ;
Kazunga, C ;
Sangaiah, R ;
Gold, A ;
Bortiatynski, J ;
Salloum, M ;
Hatcher, P .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2003, 22 (01) :40-49
[7]   Development of a catabolically significant genetic probe for polycyclic aromatic hydrocarbon-degrading Mycobacteria in soil [J].
Hall, K ;
Miller, C ;
Sorensen, DL ;
Anderson, AJ ;
Sims, RC .
BIODEGRADATION, 2005, 16 (05) :475-484
[8]   OXIDATIVE-DEGRADATION OF PHENANTHRENE BY THE LIGNINOLYTIC FUNGUS PHANEROCHAETE-CHRYSOSPORIUM [J].
HAMMEL, KE ;
GAI, WZ ;
GREEN, B ;
MOEN, MA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1832-1838
[9]   PYRENE DEGRADATION BY A MYCOBACTERIUM SP - IDENTIFICATION OF RING OXIDATION AND RING FISSION-PRODUCTS [J].
HEITKAMP, MA ;
FREEMAN, JP ;
MILLER, DW ;
CERNIGLIA, CE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (10) :2556-2565
[10]  
JIMENEZ CR, 2003, CURRENT PROTOCOLS PR, P1641