Dulaglutide Alleviates LPS-Induced Injury in Cardiomyocytes

被引:28
|
作者
Wang, Rijun [1 ,2 ]
Wang, Ning [2 ]
Han, Yuping [3 ]
Xu, Jiyao [2 ]
Xu, Zesheng [1 ]
机构
[1] Tianjin Med Univ, Dept Cardiol, Cangzhou Cent Hosp, Cangzhou 061014, Hebei, Peoples R China
[2] Shanxi Cardiovasc Hosp, Dept Cardiol, Taiyuan 030024, Shanxi, Peoples R China
[3] Shanxi Ophthalm Hosp, Dept Cornea, Taiyuan 030002, Shanxi, Peoples R China
来源
ACS OMEGA | 2021年 / 6卷 / 12期
关键词
MYOCARDIAL DEPRESSION; CK-MB; RECEPTOR; SEPSIS; EXPRESSION; DIAGNOSIS; EFFICACY; HEART; CTNI;
D O I
10.1021/acsomega.0c06326
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Background and purpose: Sepsis is a severe infection-induced disease with multiple organ failure, and sepsis-induced cardiomyopathy is a fatal condition. Inflammatory response and oxidative stress are reported to be involved in the development of sepsis-induced cardiomyopathy. Dulaglutide is a novel antidiabetic agent that is currently reported to exert an anti-inflammatory effect. The present study aims to explore the potential protective property of dulaglutide on lipopolysaccharide (LPS)-induced injury on cardiomyocytes. Methods: LPS was used to induce an in vitro injury model on cardiomyocytes. The mitochondrial reactive oxygen species (ROS) level was detected using MitoSOX red, and reduced glutathione (GSH) was measured to evaluate the status of oxidative stress in H9c2 myocardial cells. The expressions of NADPH oxidase-1 (NOX-1) and inducible nitric oxidesynthase (iNOS) were determined using real-time PCR and western blot analysis. Real-time PCR and enzyme-linked immunosorbent assay (ELISA) were both used to detect the expressions and concentrations of tumor necrosis factor-alpha, interleukin-1 beta, interleukin-17, matrix metalloproteinase-2, and matrix metalloproteinase-9 in H9c2 myocardial cells, respectively. The production of nitric oxide (NO) was measured using the Griess reagent. The levels of creatine kinase isoenzyme-MB (CK-MB) and cardiac troponin I (cTnI) were detected using ELISA. Western blot was utilized to determine the expressions of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and p-NF-kappa B p65 in H9c2 myocardial cells in the nucleus. Results: First, dulaglutide ameliorated LPS-induced oxidative stress by suppressing the production of mitochondrial ROS and elevating the level of reduced GSH, as well as downregulating NOX-1. Second, the LPS-induced cardiomyocyte injury was alleviated by dulaglutide through downregulating CK-MB and cTnI, accompanied by inhibiting iNOS expression and NO production. Lastly, the production of inflammatory factors and upregulation of MMPs induced by LPS were both significantly reversed by dulaglutide through suppressing the TLR4/Myd88/NF-kappa B signaling pathway. Conclusions: Dulaglutide alleviated LPS-induced injury in cardiomyocytes by inhibiting inflammation and oxidative stress.
引用
收藏
页码:8271 / 8278
页数:8
相关论文
共 50 条
  • [41] Hepcidin Alleviates LPS-Induced ARDS by Regulating the Ferritin-Mediated Suppression of Ferroptosis
    Jiao, Yang
    Yong, Chaoying
    Zhang, Renzi
    Qi, Di
    Wang, Daoxin
    SHOCK, 2022, 57 (06): : 274 - 281
  • [42] Stabilization of Brain Mast Cells Alleviates LPS-Induced Neuroinflammation by Inhibiting Microglia Activation
    Dong, Hongquan
    Wang, Yiming
    Zhang, Xiaojun
    Zhang, Xiang
    Qian, Yanning
    Ding, Haixia
    Zhang, Shu
    FRONTIERS IN CELLULAR NEUROSCIENCE, 2019, 13
  • [43] Cecropin A Alleviates LPS-Induced Oxidative Stress and Apoptosis of Bovine Endometrial Epithelial Cells
    Zhao, Yu
    Zhang, Yang
    Sun, Mingkun
    Li, Bowen
    Li, Yuqiong
    Hua, Song
    ANIMALS, 2024, 14 (05):
  • [44] Micromeria congesta Alleviates LPS-Induced Inflammation, Apoptosis, Oxidative Stress and DNA Damage in Rat Heart and Kidneys
    Dortbudak, Muhammet Bahaeddin
    Demircioglu, Muhammed
    Kapucuk, Fuat Serkan
    VETERINARY MEDICINE AND SCIENCE, 2025, 11 (02)
  • [45] miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway
    Dong, Wei
    Chen, Jin
    Wang, Yadong
    Weng, Junfei
    Du, Xingxiang
    Fang, Xu
    Liu, Wenyu
    Long, Tao
    You, Jiaxiang
    Wang, Wensheng
    Peng, Xiaoping
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2024, 479 (04) : 929 - 940
  • [46] Leptin Downregulates LPS-Induced Lung Injury: Role of Corticosterone and Insulin
    Landgraf, Maristella A.
    Silva, Reinaldo C.
    Correa-Costa, Matheus
    Hiyane, Meire I.
    Carvalho, Maria Helena C.
    Landgraf, Richardt G.
    Camara, Niels O. S.
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2014, 33 (03) : 835 - 846
  • [47] Protective effect of oxytocin on LPS-induced acute lung injury in mice
    An, Xiaona
    Sun, Xiaotong
    Hou, Yonghao
    Yang, Xiaomei
    Chen, Hongli
    Zhang, Peng
    Wu, Jianbo
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [48] Lactoferrin moderates LPS-induced hypotensive response and gut injury in rats
    Doursout, Marie-Francoise
    Horton, Haley
    Hoang, Linh
    Liang, Yangyan
    Hwang, Shen-An
    Boyd, Sydney
    Actor, Jeffrey K.
    Kruzel, Marian L.
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2013, 15 (02) : 227 - 231
  • [49] Tectorigenin inhibits the inflammation of LPS-induced acute lung injury in mice
    Ma Chun-Hua
    Liu Ji-Ping
    Qu Rong
    Ma Shi-Ping
    CHINESE JOURNAL OF NATURAL MEDICINES, 2014, 12 (11) : 841 - 846
  • [50] A novel role of endocan in alleviating LPS-induced acute lung injury
    Zhang, Xiaolong
    Zhuang, Rong
    Wu, Haiya
    Chen, Jie
    Wang, Fangyan
    Li, Guoping
    Wu, Chengyun
    LIFE SCIENCES, 2018, 202 : 89 - 97