First report of Escherichia coli O157:H7 in ready-to-eat sushi

被引:8
|
作者
Ramires, T. [1 ]
Iglesias, M. A. [2 ]
Vitola, H. S. [1 ]
Nuncio, A. S. P. [1 ]
Kroning, I. S. [1 ]
Kleinubing, N. R. [1 ]
Fiorentini, A. M. [1 ]
da Silva, W. P. [1 ,2 ]
机构
[1] Univ Fed Pelotas, Sci & Agroind Technol Dept, Pelotas, RS, Brazil
[2] Univ Fed Pelotas, Technol Dev Ctr, Biotechnol Unit, Pelotas, RS, Brazil
关键词
biofilm; E. coli O157:H7; ready-to-eat; STEC; sushi;
D O I
10.1111/jam.14456
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: The aim of this study was to evaluate the microbiological quality of commercially prepared ready-to-eat (RTE) sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella ssp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form biofilms on stainless steel. Methods and Results: There were four sampling events in seven establishments, totalling 28 pools of sushi samples. Mean AMB counts ranged between 5.2 and 7.7 log CFU per gram. The enumeration of TC varied between 2.1 and 2.7 log MPN per gram. Salmonella ssp. were not detected, and one sample was positive for E. coli and was identified as E. coli O157:H7. To the best of our knowledge, this is the first report of E. coli O157:H7 in sushi samples in the world literature. This isolate presented virulence genes stx1, stx2, eae and hlyA. It was also susceptible to 14 antimicrobials tested and had the ability to form biofilms on stainless steel. Conclusions: There is a need to improve the good hygiene practices adopted in establishments selling sushi in the city of Pelotas, Brazil. In addition, the isolated E. coli O157:H7 carries a range of important virulence genes being a potential risk to consumer health, as sushi is a RTE food. This isolate also presents biofilm formation ability, therefore, may trigger a constant source of contamination in the production line of this food. Significance and Impact of the Study: The increase in the consumption of sushi worldwide attracts attention regarding the microbiological point of view, since it is a ready-to-eat food. To our knowledge, this was the first time that E. coli O157:H7 was identified in sushi samples.
引用
收藏
页码:301 / 309
页数:9
相关论文
共 50 条
  • [41] Identification and Prevalence of Escherichia coli and Escherichia coli O157: H7 in Foods
    Rotar, Ancuta M.
    Semeniuc, Cristina Anamaria
    Apostu, Sorin
    Pop, Carmen
    Duma, Mihaela
    Suharoschi, Ramona
    Giura, Larisa
    BULLETIN OF UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE CLUJ-NAPOCA-FOOD SCIENCE AND TECHNOLOGY, 2013, 70 (02) : 139 - 140
  • [42] Effect of X-ray treatments on inoculated Escherichia coli O157: H7, Salmonella enterica, Shigella flexneri and Vibrio parahaemolyticus in ready-to-eat shrimp
    Mahmoud, Barakat S. M.
    FOOD MICROBIOLOGY, 2009, 26 (08) : 860 - 864
  • [43] Performance of the ImmunoCard STAT!: E. coli O157:H7 test for detection of Escherichia coli O157:H7 in stools
    Mackenzie, A
    Orrbine, E
    Hyde, L
    Benoit, M
    Chan, F
    Park, C
    Alverson, J
    Lembke, A
    Hoban, D
    Kennedy, W
    JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) : 1866 - 1868
  • [44] Escherichia coli O157:H7 causing hemorrhagic colitis
    Hettiarachchi, Irasha Thulani
    Hegde, Manish
    Planner, Andrew Charles
    John, Lawrence
    GASTROINTESTINAL ENDOSCOPY, 2012, 75 (03) : 674 - 675
  • [45] Genomic anatomy of Escherichia coli O157:H7 outbreaks
    Eppinger, Mark
    Mammel, Mark K.
    Leclerc, Joseph E.
    Ravel, Jacques
    Cebula, Thomas A.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (50) : 20142 - 20147
  • [46] Thermal destruction of Escherichia coli O157:H7 in hamburger
    Juneja, VK
    Snyder, OP
    Williams, AC
    Marmer, BS
    JOURNAL OF FOOD PROTECTION, 1997, 60 (10) : 1163 - 1166
  • [47] Vaccination of Cattle against Escherichia coli O157: H7
    Smith, David R.
    MICROBIOLOGY SPECTRUM, 2014, 2 (06):
  • [48] Identification of Escherichia coli O157:H7 with Oligonucleotide Arrays
    H. Liu
    H. Wang
    Z. Shi
    Q. Liu
    J. Zhu
    N. He
    H. Wang
    Z. Lu
    Bulletin of Environmental Contamination and Toxicology, 2003, 71 : 0826 - 0832
  • [49] Enterohaemorrhagic Escherichia coli O157:H7:: a red herring?
    Bettelheim, KA
    JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (02) : 201 - 202
  • [50] Transmission and control of Escherichia coli O157:H7 -: A review
    Bach, SJ
    McAllister, TA
    Veira, DM
    Gannon, VPJ
    Holley, RA
    CANADIAN JOURNAL OF ANIMAL SCIENCE, 2002, 82 (04) : 475 - 490