Preactivated and disaggregated shape-changed platelets protect kidney against from ischemia-reperfusion injury in rat through attenuating inflammation reaction

被引：11

作者：

Chen, Yen-Ta ^{[1
,2
]}

Yang, Chih-Chao ^{[3
]}

Lin, Kun-Chen ^{[4
]}

Chen, Kuan-Hung ^{[4
]}

Sung, Pei-Hsun ^{[5
]}

Shao, Pei-Lin ^{[6
,7
]}

Li, Yi-Chen ^{[5
]}

Chiang, John Y. ^{[9
]}

Yip, Hon-Kan ^{[2
,5
,6
,7
,8
]}

机构：

[1] Chang Gung Univ, Kaohsiung Chang Gung Mem Hosp, Dept Surg, Div Urol,Coll Med, Kaohsiung, Taiwan

[2] Kaohsiung Chang Gung Mem Hosp, Ctr Shockwave Med & Tissue Engn, Kaohsiung, Taiwan

[3] Chang Gung Univ, Kaohsiung Chang Gung Mem Hosp, Dept Internal Med, Div Nephrol,Coll Med, Kaohsiung, Taiwan

[4] Chang Gung Univ, Kaohsiung Chang Gung Mem Hosp, Dept Anesthesiol, Coll Med, Kaohsiung, Taiwan

[5] Chang Gung Univ, Kaohsiung Chang Gung Mem Hosp, Dept Internal Med, Div Cardiol,Coll Med, Kaohsiung, Taiwan

[6] Asia Univ, Dept Nursing, Taichung, Taiwan

[7] China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung, Taiwan

[8] Kaohsiung Chang Gung Mem Hosp, Inst Translat Res Biomed, Kaohsiung 83301, Taiwan

[9] Natl Sun Yat Sen Univ, Dept Comp Sci & Engn, Kaohsiung, Taiwan

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2019年 / 13卷 / 12期

关键词：

acute ischemia-reperfusion injury; cell apoptosis; inflammation; kidney injury;

D O I：

10.1002/term.2960

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP), 3 (IR only), and 4 (IR + PreD-SCP). By 72 hr after IR procedure, the circulatory levels of creatinine, blood urine nitrogen and inflammatory biomarkers (interleukin [IL]-6/tumor necrosis factor [TNF]-alpha), and ratio of urine protein to urine creatinine were significantly higher in Group 3 than in other groups and significantly higher in Group 4 than in Groups 1 and 2, but they showed no different between Groups 1 and 2 (all p <.001). The microscopic findings showed that the expressions of kidney injury score, cellular inflammation (MMP-9/CD14//F4/80), and fibrotic area were identical to the circulatory inflammation, whereas the integrity of podocyte components (ZO-1/synaptopodin/podocin) exhibited an opposite to circulatory inflammation among the four groups (all p < .0001). The protein expressions of inflammatory (TNF-alpha/IL-1 beta/NF-kappa B/iNOS/TRAF6/MyD88/TLR-4), apoptotic/cell death (mitochondrial Bax/cleaved caspase-3/p-53), oxidized protein, mitogen-activated protein kinase family (p-38/p-JNK/p-c-JUN), and mitochondrial-damaged biomarkers displayed a similar pattern, whereas the antiapoptotic (Bcl-2/Bcl-XL) and integrity of mitochondrial biomarkers followed an opposite trend to circulatory inflammation among the four groups (all p < .001). PreD-SCP therapy effectively protected the kidney against IR injury.

引用

页码：2155 / 2168

页数：14

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