Aqueous phase separation in giant vesicles

被引：83

作者：

Helfrich, MR ^{[1
]}

Mangeney-Slavin, LK ^{[1
]}

Long, MS ^{[1
]}

Djoko, Y ^{[1
]}

Keating, CD ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 45期

关键词：

D O I：

10.1021/ja028157+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the synthesis and initial characterization of ∼10 μm diameter lipid vesicles that contain two distinct aqueous phases. The aqueous two-phase system is a dextran/poly(ethylene glycol) solution that exhibits temperature-dependent phase behavior. Vesicles were prepared above the phase transition temperature of the polymer solution. Upon cooling to room temperature, the polymer solution phase separated both within the vesicles and in the bulk solution. The location of poly(ethylene glycol)-rich and dextran-rich phases was determined by fluorescence microscopy. These structures are exciting in that they enable for the first time the interior volume of liposomes to be structured. Copyright © 2002 American Chemical Society.

引用

页码：13374 / 13375

页数：2

共 50 条

[41] Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol [J].

Veatch, SL ;

Keller, SL .

BIOPHYSICAL JOURNAL, 2003, 85 (05) :3074-3083

[42] Phase separation of aqueous solution of methylcellulose [J].

C. Chevillard ;

M. A. V. Axelos .

Colloid and Polymer Science, 1997, 275 :537-545

[43] PHASE SEPARATION IN AQUEOUS COLLOIDAL SYSTEMS [J].

KOETS, P .

REPORTS ON PROGRESS IN PHYSICS, 1945, 10 :129-140

[44] Biomimetic microcompartmentalization by aqueous phase separation [J].

Keating, Christine .

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 250

[45] PHASE SEPARATION IN AN AQUEOUS QUATERNARY SYSTEM [J].

EDMOND, E ;

OGSTON, AG .

BIOCHEMICAL JOURNAL, 1970, 117 (01) :85-&

[46] MECHANISM OF AQUEOUS PHASE-SEPARATION [J].

VANOSS, CJ ;

GOOD, RJ ;

CHAUDHURY, MK .

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 195 :152-IEC

[47] Phase separation of aqueous solution of methylcellulose [J].

Chevillard, C ;

Axelos, MAV .

COLLOID AND POLYMER SCIENCE, 1997, 275 (06) :537-545

[48] Aqueous two-phase systems for protein separation: Phase separation and applications [J].

Asenjo, Juan A. ;

Andrews, Barbara A. .

JOURNAL OF CHROMATOGRAPHY A, 2012, 1238 :1-10

[49] Oxidized phospholipids induce phase separation in lipid vesicles [J].

Megli, FM ;

Russo, L ;

Sabatini, K .

FEBS LETTERS, 2005, 579 (21) :4577-4584

[50] Deformation-induced phase separation of active vesicles [J].

Jin, Yi-yang ;

Jin, Yan ;

Shi, Zi-xuan ;

Tian, Wen-de ;

Zhang, Tian-hui ;

Chen, Kang .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2024, 26 (37) :24699-24708

← 1 2 3 4 5 →