Aqueous phase separation in giant vesicles

被引：79
作者：
Helfrich, MR [1 ]
Mangeney-Slavin, LK [1 ]
Long, MS [1 ]
Djoko, Y [1 ]
Keating, CD [1 ]
机构：
[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 45期
关键词：
D O I：
10.1021/ja028157+
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the synthesis and initial characterization of ∼10 μm diameter lipid vesicles that contain two distinct aqueous phases. The aqueous two-phase system is a dextran/poly(ethylene glycol) solution that exhibits temperature-dependent phase behavior. Vesicles were prepared above the phase transition temperature of the polymer solution. Upon cooling to room temperature, the polymer solution phase separated both within the vesicles and in the bulk solution. The location of poly(ethylene glycol)-rich and dextran-rich phases was determined by fluorescence microscopy. These structures are exciting in that they enable for the first time the interior volume of liposomes to be structured. Copyright © 2002 American Chemical Society.
引用
收藏
页码：13374 / 13375
页数：2
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